Dri-Lyse: Stabilised Bacteriophage Formulations for Sustainable Plant Protection and "Foodprint" Reduction

Dri-Lyse：用于可持续植物保护和减少“足迹”的稳定噬菌体配方

• 批准号: 48477
• 金额: $ 38.76万
• 依托单位: APS BIOCONTROL LIMITED
• 依托单位国家: 英国
• 项目类别: Study
• 财政年份: 2020
• 资助国家: 英国
• 起止时间: 2020 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=48477
• 关键词: Dri; Lyse; Stabilised; Bacteriophage; Formulations

Bacterial pathogens of fresh produce are responsible for substantial losses through disease, damage and failure to meet market specifications, with no specific controls. Diseased crop and waste-food disposal, plus additional agricultural land and water required to achieve sufficient marketable yields all add up to increase the carbon footprint of food production ("foodprint") and more efficient agricultural systems, with less wastage are a priority to meet UK and EU targets of increased food productivity and reduced waste.**Bacteriophage** are viruses; they are the most abundant organisms on earth. As naturally-occurring bacterial enemies, they are attracting considerable attention as targeted agricultural antibacterial-control products. They are completely safe for the consumer, highly host-specific and have zero environmental or non-target impacts. APS Biocontrol has made good progress in demonstrating their potential in reducing bacterial plant disease but product commercial viability is limited; liquid products have limited thermal-stability, requiring low-temperature storage and transport, which impacts on their cost, shelf-life and storage. The project's **key objective** is to address these limitations, developing thermally-stable, dry bacteriophage products (_Dri-Lyse);_ achieved through working with Stabilitech BioPharma, an established UK developer of vaccines and therapeutics, with platform technologies to significantly enhance product stability, including for viruses and based around unique, safe, small molecules ("excipients") which are naturally present in foods. In pilot studies, their technology has given promising thermal stabilisation of sample bacteriophage, forming the background to this project.The project will focus on the UK and wider European potato market; through previous Innovate UK support APS has developed an effective bacteriophage mix ("BPSRE") against bacterial-induced "Blackleg" of potatoes, responsible for £50M UK total losses p.a. and £750M worldwide. APS has also developed a liquid bacteriophage product (Biolyse) to target the downstream losses caused by this disease; most frequently realised in the pack house as rotting potatoes. The focus of the **technical Innovation** will be creating an optimised excipient mix to maintain the activity of the BPSRE mix.Our vision is to switch from liquid bacteriophage mixes to a tableted/powered form, rehydratable at the point of use. Benefits will be a longer shelf life, making the products more manufacturable. Also, removing the need for chilled storage and transport, they become more attractive to the customer and significantly better placed to address export markets for business growth and globalisation. Once proven in the potato market, the longer vision is to address other high-value fresh-produce markets, both in the UK and globally.

新鲜农产品的细菌病原体通过疾病、损害和未能达到市场规格而造成的重大损失，没有具体的控制措施。为了实现英国和欧盟提高粮食生产率和减少浪费的目标，病态作物和废物处理，加上实现足够的适销对路产量所需的额外农业土地和水，所有这些加起来都会增加粮食生产的碳足迹(“粮食足迹”)和更高效的农业系统。**噬菌体**是病毒；它们是地球上最丰富的生物体。作为自然产生的细菌天敌，它们作为有针对性的农业抗菌控制产品正受到相当大的关注。它们对消费者是完全安全的，高度特定于主机，对环境或非目标影响为零。APS生防菌在减少细菌性植物病害方面取得了很好的进展，但产品的商业可行性有限；液体产品的热稳定性有限，需要低温储存和运输，这影响了它们的成本、货架期和储存。该项目的**关键目标**是为了解决这些限制，开发耐热、干燥的噬菌体产品(_Dri-Lyse)；_通过与英国著名的疫苗和治疗药物开发商Stbilitech BioPharma合作实现_，利用平台技术显著提高产品的稳定性，包括针对病毒和基于天然存在于食品中的独特、安全、小分子(“赋形剂”)。在初步研究中，他们的技术为样本噬菌体提供了有希望的热稳定性，构成了这个项目的背景。该项目将专注于英国和更广泛的欧洲土豆市场；通过之前的Innovate UK支持，APS已经开发出一种有效的噬菌体混合物(BPSRE)，可以对抗细菌诱导的土豆“黑腿”，每年造成英国5000万英镑的总损失。和全球7.5亿GB。APS还开发了一种液体噬菌体产品(Biolyse)，以针对这种疾病造成的下游损失；在包装厂最常实现的是腐烂的土豆。**技术创新**的重点将是创造一种优化的赋形剂混合物，以保持BPSRE混合物的活性。我们的愿景是从液体噬菌体混合物转变为片状/粉末状，可在使用时补充水分。好处是保质期更长，使产品更易于制造。此外，消除了对冷藏存储和运输的需求，它们对客户变得更具吸引力，并更好地满足出口市场的业务增长和全球化。一旦在土豆市场得到证实，长远的愿景是解决英国和全球的其他高价值新鲜农产品市场。