PIEZOELECTRIC IMMUNOSENSOR FOR DIAGNOSIS OF TB

用于诊断结核病的压电免疫传感器

• 批准号: 2070921
• 负责人: STEVEN C PERLAKY
• 金额: $ 7.12万
• 依托单位: S AND P MEDICAL, INC.
• 依托单位国家: 美国
• 财政年份: 1994
• 资助国家: 美国
• 起止时间: 1994-01-01 至 1994-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2070921
• 关键词: Mycobacterium tuberculosis; antibacterial antibody; bacterial antigens; biosensor device; body fluids; cerebrospinal fluid; communicable disease diagnosis; diagnostic respiratory lavage; electrodes; microorganism culture; monoclonal antibody; sputum; synovial fluid; tuberculosis; vapor

The long-term objective of the proposed research is to develop a rapid method for the diagnosis of tuberculosis. A piezoelectric biosensor employing antibodies to soluble mycobacterial antigens will be used to detect these specific antigens and/or whole mycobacteria. Detection of mycobacterial antigens in a liquid phase will allow the diagnosis of mycobacterial infection in biological fluids such as sputum, bronchoalveolar lavage fluid, cerebral spinal fluid and synovial fluid. Ultimately, gas or vapor phase detection of mycobacterial antigens by a piezo-immunosensor would allow rapid diagnosis of pulmonary tuberculosis by detection of mycobacterial antigens in expired human breath. The feasibility of this novel approach to the diagnosis of tuberculosis will be tested using monoclonal and polyclonal antibodies to specific mycobacterial antigens. The antibodies will be coupled to gold electrodes on AT-cut 9 MHzquartz crystals. The resulting piezoelectric- immunosensor will be tested for its ability to detect mycobacterial antigens in culture supernatants and in solutions of antigen vaporized by a nebulizer.

拟议研究的长期目标是开发一种快速的 结核病的诊断方法 压电生物传感器 使用可溶性分枝杆菌抗原的抗体将用于 检测这些特异性抗原和/或整个分枝杆菌。 检测 液相中的分枝杆菌抗原将允许诊断 生物流体如痰中的分枝杆菌感染， 支气管肺泡灌洗液、脑脊液和滑液。 最后，通过气相或气相色谱法检测分枝杆菌抗原， 压电免疫传感器可快速诊断肺结核 通过检测呼出的人类呼吸中的分枝杆菌抗原。 这种新方法诊断结核病的可行性 将使用特异性的单克隆和多克隆抗体进行检测 分枝杆菌抗原 抗体会和黄金结合 AT切割9 MHz石英晶体上的电极。 由此产生的压电- 将测试免疫传感器检测分枝杆菌能力 培养物上清液和蒸发的抗原溶液中的抗原 通过喷雾器