PIEZOELECTRIC IMMUNOSENSOR FOR DIAGNOSIS OF TB

拟议研究的长期目标是快速开发一种 结核病的诊断方法。一种压电式生物传感器 使用针对可溶性分枝杆菌抗原的抗体将用于 检测这些特定的抗原和/或整个分枝杆菌。检测 液体中的分枝杆菌抗原将允许诊断 痰等生物体液中的分枝杆菌感染， 支气管肺泡灌洗液、脑脊液和滑液。 最终，分枝杆菌抗原的气相或气相检测 压电式免疫传感器可用于肺结核的快速诊断 通过检测呼气中的分枝杆菌抗原。 这种新方法在结核病诊断中的可行性 将使用单抗和多克隆抗体进行检测，以识别 分枝杆菌抗原。抗体将偶联到金子上 AT-Cut 9 MHz石英晶体上的电极。由此产生的压电式- 将测试免疫传感器检测分枝杆菌的能力 培养上清液和挥发抗原溶液中的抗原 用喷雾器喷雾。

The long-term objective of the proposed research is to develop a rapid method for the diagnosis of tuberculosis. A piezoelectric biosensor employing antibodies to soluble mycobacterial antigens will be used to detect these specific antigens and/or whole mycobacteria. Detection of mycobacterial antigens in a liquid phase will allow the diagnosis of mycobacterial infection in biological fluids such as sputum, bronchoalveolar lavage fluid, cerebral spinal fluid and synovial fluid. Ultimately, gas or vapor phase detection of mycobacterial antigens by a piezo-immunosensor would allow rapid diagnosis of pulmonary tuberculosis by detection of mycobacterial antigens in expired human breath. The feasibility of this novel approach to the diagnosis of tuberculosis will be tested using monoclonal and polyclonal antibodies to specific mycobacterial antigens. The antibodies will be coupled to gold electrodes on AT-cut 9 MHzquartz crystals. The resulting piezoelectric- immunosensor will be tested for its ability to detect mycobacterial antigens in culture supernatants and in solutions of antigen vaporized by a nebulizer.