CELL ADHESION MOLECULES IN CARDIOGENESIS

心肌发生中的细胞粘附分子

• 批准号: 2218725
• 负责人: MICHIKO WATANABE
• 金额: $ 19.18万
• 依托单位: CASE WESTERN RESERVE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1988
• 资助国家: 美国
• 起止时间: 1988-07-01 至 1998-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2218725
• 关键词: aminosugar; carcinogenesis; cell cell interaction; chick embryo; gap junctions; growth /development; heart cell; heart conduction system; heart electrical activity; histogenesis; immunocytochemistry; myocardium; neural cell adhesion molecules

DESCRIPTION (taken from applicants abstract): The role of polysialated NCAM in the normal development of the cardiac conduction system will be investigated in the chicken embryo model. The neural cell adhesion molecule NCAM and its polysialic acid moiety are modulators of cell-cell interactions found in the developing heart. Up and down regulation of these components during neural and skeletal muscle development is critical for creating the balance that results in normal morphogenetic patterns. In previous studies, the applicant has demonstrated that NCAM and PSA are regulated in their expression in developing heart tissue. She has also shown that perturbation of NCAM or PSA alters the function of gap junctions which couple myocardial cells and PSA-rich NCAM is found preferentially on ventricular conduction tissue. These findings has led the investigator to propose that NCAM and PSA are acting as they do on neuronal processes to allow plasticity in interactions of myocardial cells, particularly those of conduction tissue during cardiogenesis. The aims of this study are: 1) to refine this hypothesis by determining the PSA distribution in regions and stages not yet studied in detail; and 2) to test their hypothesis by enzymatically removing PSA from the embryonic heart in ovo and assessing the consequences to the morphology, biochemistry and electrophysiology of the conduction system.

描述（摘自申请人摘要）：聚唾液酸化的作用 NCAM在心脏传导系统的正常发育中会 在鸡胚胎模型中进行了研究。神经细胞粘附 分子 NCAM 及其聚唾液酸部分是细胞间的调节剂 在发育中的心脏中发现相互作用。上下调节 这些成分在神经和骨骼肌发育过程中是 对于创造导致正常形态发生的平衡至关重要 模式。在之前的研究中，申请人已经证明 NCAM 和 PSA 在发育中的心脏组织中的表达受到调节。 她还表明，NCAM 或 PSA 的扰动会改变功能 耦合心肌细胞和富含 PSA 的 NCAM 的间隙连接是 优先发现于心室传导组织。这些发现 导致调查人员提出 NCAM 和 PSA 正在按照他们的方式行事 对神经元过程进行作用，以允许相互作用的可塑性 心肌细胞，特别是传导组织的心肌细胞 心脏发生。本研究的目的是：1）完善这一假设 通过确定尚未确定的地区和阶段的 PSA 分布 详细研究； 2）通过酶法检验他们的假设 从卵内胚胎心脏中去除 PSA 并评估 对形态学、生物化学和电生理学的影响 传导系统。