CARDIAC PROGENITOR CELL DIFFERENTIATION MOLECULAR ANAL

说明(改编自申请人的摘要)： 目前的研究是为了了解建立的机制 和禽类心肌细胞谱系的分化 胚胎。使用此模型系统，申请人先前已 演示了心脏特异的激活时间 转录机制，早期中胚层细胞的分化 在文化中解释时来自心脏形成区，以及 心肌细胞增强因子心脏特异成员的表达 2(MEF2)家系。拟议的调查旨在：(1) 心脏特异性调控候选因子的筛选和克隆 级联，使用消减杂交技术；(2)鉴定和 克隆表达先于或先于MEF2家族成员的基因 与心源性命运的分配相吻合，并评估他们的 在确定和维持心肌细胞谱系中的作用； 以及(3)确定其产物能力的候选基因 有助于确定和/或维持心源性疾病 血统。

DESCRIPTION (adapted from the applicant's abstract): The purpose of the present study is to understand the mechanisms underlying establishment and differentiation of the cardiac muscle cell lineage in the avian embryo. Using this model system, the applicant has previously demonstrated the timing of activation of cardiac-specific transcriptional machinery, the differentiation of early mesodermal cells from the heart forming area when explanted in culture, and the expression of cardiac-specific members of the myocyte enhancer factor 2 (MEF2) family. The proposed investigation is designed to: (1) identify and clone candidate factors of the cardiac-specific regulatory cascade, using subtractive hybridization techniques; (2) identify and clone genes for members of the MEF2 family whose expression precedes or coincides with the assignment of cardiogenic fate and to assess their role in the determination and maintenance of the cardiac cell lineage; and (3) characterize candidate genes for the ability of their products to contribute to the determination and/or maintenance of the cardiogenic lineage.