SPECIATION OF HDL AND LDL

该项目的目的是利用最近的重大进展， 了解血浆脂蛋白的结构-功能关系 描述女性的生物过程， 易患冠状动脉疾病或提供保护。 通过识别新的风险因素， 识别高风险的个人，还可以提供 为缓解过程而设计的新治疗策略的基础 或者增强那些起保护作用的。 然而，极端集中化似乎扰乱了当地的建筑， 高密度脂蛋白（HDL），一种新开发的非 变性免疫吸附允许分离七到八个 离散物种这些新发现物种的数量分布图 将在200名育龄和绝经后妇女中进行测量。 将研究各个物种的功能作用， 胆固醇酯转移到受体脂蛋白， 对胆固醇回收途径至关重要。转让不足 胆固醇酯通过HDL与低密度脂蛋白（LDL）结合可能是 高密度LDL现象，在一些研究中与 冠心病 本课题将探讨HDL与 物种分布与这种现象有关。 LDL的氧化可能是关键 动脉性心脏病的发展。 显著 已经证明HDL对该过程的抑制。 的目的 这项研究是确定所有HDL种类负责 这种抗氧化作用及其作用机制。

The aim of this project is to take advantage of major recent advances in knowledge of the structure-function relationships of plasma lipoproteins to characterize, in women, biological processes that are likely to predispose to coronary disease or to confer protection against it. Such knowledge, by identifying new risk factors, could lead to early identification of individuals at high risk and could also provide the basis for novel strategies of treatment designed to mitigate processes that contribute to atherogenesis, or to enhance those that are protective. Whereas ultracentrifugation appears to derange the native architecture of high density lipoproteins (HDL), a newly developed strategy of non- denaturing immunosorption permits the separation of seven to eight discrete species. Quantitative profiles of these newly discovered species will be measured in two hundred reproductive age and postmenopausal women. Functional roles of the individual species will be studied with respect to the transfer of cholesteryl esters to acceptor lipoproteins, a step critical to the cholesterol retrieval pathway. Deficient transfer of cholesteryl esters to low density lipoproteins (LDL) by HDL may underlie the phenomenon of hyperdense LDL, related in some studies to risk of coronary disease. This project will explore the relationship of HDL species distribution to this phenomenon. Oxidation of LDL may be critical to the development of arteriosclerotic heart disease. Significant inhibition of the process by HDL has been demonstrated. An objective of this study is the identification of all the HDL species responsible for this antioxidant effect and the mechanisms by which they act.