BIOCHEMICAL RESPONSE OF OSTEOBLASTS TO MATERIAL SURFACES

该研究计划将使用细胞培养模型来检查反应 将骨细胞转化为生物材料。 细胞将在冠状细胞上培养- 放电-聚苯乙烯或在已经过电镀涂层的盘上， 商业纯钛，以评估是否暴露于钛 改变细胞对生长因子的反应。 三个假设将是 测试：（1）当细胞增殖和基质合成发生变化时， 成骨样细胞在钛片上生长;（2）基质囊泡和基质 当这些细胞在Ti上培养时，钙化发生改变;以及（3） 重组人血管内皮细胞对基质合成和钙化的调控 转化生长因子-β 1（TGF β）在细胞生长时发生改变。 培养的Ti。 为了检验这些假设的有效性，以下 目的：（1）比较细胞增殖和基质 胎鼠颅骨（FRC）细胞在常规培养板上的合成 电晕放电-聚苯乙烯（塑料）与钛涂层表面;（2） 表征基质膀胱结构和功能， 在塑料与钛涂层表面上培养的FRC细胞钙化;以及 (3)为了确定在Ti上培养是否改变FRC细胞对 TGF β

The research program will use a cell culture model to examine the response of bone cells to biomaterials. Cells will be cultured on corona- discharged-polystyrene or on dishes which have been sputter-coated with commercially pure titanium in order to assess whether exposure to Ti alters cellular response to growth factors. Three hypotheses will be tested: (1) Cell proliferation and matrix synthesis are altered when osteoblast-like cells are grown on Ti; (2) Matrix vesicle and matrix calcification are altered when these cells are cultured on Ti; and (3) Regulation of matrix synthesis and calcification by recombinant human transforming growth factor-beta1 (TGFbeta) are altered when cells are cultured by Ti. To test the validity of these hypotheses, the following aims are proposed: (1) To compare cellular proliferation and matrix synthesis of fetal rat calvarial (FRC) cells cultured on conventional corona-discharged-polystyrene (plastic) v. ti-coated surfaces; (2) To characterize matrix vesical structure and function and matrix calcification by FRC cells cultured on plastic v. Ti-coated surfaces; and (3) To determine whether culture on Ti alters FRC cell response to TGFbeta.