APICAL COMPLEX ORGANELLES AND PROTEINS OF BABESIA BOVIS

牛巴贝斯虫顶端复合体细胞器和蛋白质

• 批准号: 2057109
• 负责人: SUSAN C DOWLING
• 金额: $ 6.02万
• 依托单位: WASHINGTON STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1993
• 资助国家: 美国
• 起止时间: 1993-06-01 至 1996-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2057109
• 关键词: babesiosis; cellular pathology; disease /disorder prevention /control; microorganism antigen; organelles; zoonosis

Research Proposal: Babesia bovis is one of the most significant parasites of cattle worldwide. Morbidity and mortality associated with babesiosis and other important apicomplexan diseases, such as malaria, coccidiosis and theileriosis, has prompted intensive efforts to improve currently inadequate control methods. Possible novel targets for control are the apical complex organelles that are unique to the Apicomplexa and perform important, although incompletely understood, functions in their life cycles. Ultrastructural evidence strongly implicates the involvement of the apical complex in host cell invasion. In addition, antibody to several apical complex proteins can block erythrocyte invasion. This has yet to be demonstrated for B. bovis. In the proposed studies, 1) apical complex organelles will be isolated, and 2) polyclonal and monoclonal antibody reagents to apical complex organelles and proteins generated, respectively. These reagents will be used in future experiments to test the hypothesis that antibody to apical complex proteins will inhibit erythrocyte invasion by B. bovis merozoites. This strategy is designed to rapidly identify apical complex proteins which function in invasion. Once identified, the molecular details of their roles in the invasion process can be investigated in long-term studies. Candidate: The candidate is a veterinarian completing the clinical service component of a residency in veterinary anatomic pathology, and is a candidate for the Ph.D. in veterinary science . The experiments described in this proposal will constitute Specific Aims 1 and 2 of her doctoral research. The results will provide directions for the long-term objective of elucidating the mechanisms of host cell entry by apicomplexan parasites. Environment: The Department of Veterinary Microbiology and Pathology is recognized for contributions to the molecular biology and immunology of veterinary infectious diseases, and has facilities equipped for performing technologically current research in these areas. Collaborative research in bovine babesiosis figures prominently among departmental projects. The primary sponsor has experience in the molecular biology of protozoan and metazoan parasites, including intracellular parasite development, and isolation and characterization of immunoprotective surface proteins.

研究建议：牛Bababybovis是最重要的寄生虫之一 全世界的牛与巴贝虫病相关的发病率和死亡率 以及其他重要的顶复门疾病，如疟疾、球虫病 和泰勒虫病，促使人们加大努力，以改善目前 控制方法不足。 可能的新的控制目标是 顶端复杂的细胞器，是独特的顶复门，并执行 重要的，虽然不完全理解，功能在他们的生活 自行车. 超微结构证据强烈暗示了 在宿主细胞侵入中顶端复合体。 此外，抗 几种顶端复合物蛋白可以阻断红细胞侵入。这 尚未针对B进行演示。牛 在拟定的研究中，1）顶侧 分离复杂的细胞器，2）多克隆和单克隆 抗体试剂顶端复杂的细胞器和蛋白质产生， 分别 这些试剂将在未来的实验中用于测试 顶端复合体蛋白的抗体将抑制 B侵入红细胞。牛裂殖子 该战略旨在 以快速鉴定在侵袭中起作用的顶端复合物蛋白。 一旦确定，它们在入侵中的分子细节 可以在长期研究中进行研究。 候选人：候选人是一名完成临床试验的兽医。 兽医解剖病理学住院服务的组成部分，是 博士候选人在兽医科学中。实验 本提案所述内容将构成其具体目标1和2 博士研究。 结果将为长期提供方向 目的是阐明宿主细胞进入的机制， 顶复寄生虫 环境：兽医微生物学和病理学系是 因其在分子生物学和免疫学方面的贡献而受到认可。 兽医传染病，并配备了设施， 在这些领域进行技术研究。 在牛巴贝虫病的合作研究中， 部门项目。 主要申办者在以下方面具有经验： 原生动物和后生动物寄生虫的分子生物学，包括 细胞内寄生虫的发育、分离和表征 免疫保护性表面蛋白。