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STRUCTURAL AND DYNAMIC STUDIES OF PROTEINS BY NMR

通过 NMR 对蛋白质进行结构和动力学研究

基本信息

批准号：
2084027
负责人：
ROBERT A BECKMAN
金额：
$ 8.78万
依托单位：
UNIVERSITY OF MICHIGAN AT ANN ARBOR
依托单位国家：
美国
项目类别：
财政年份：
1989
资助国家：
美国
起止时间：
1989-07-01 至 1995-06-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/2084027
关键词：
avian sarcoma virus cow enzyme mechanism enzyme structure lens proteins nuclear magnetic resonance spectroscopy peptidases protein folding protein structure protein structure function radiotracer stop flow technique thermodynamics training virus protein

项目摘要

The proposed Physician Scientist training program will train the candidate in modern nuclear magnetic resonance (NMR) methods for evaluation of structure and dynamics of macromolecules, particularly proteins, and will deepen his appreciation of protein structure-function relationships. The candidate will later apply these techniques, together with his knowledge of enzyme kinetics, to the study of medically significant molecules within his chosen clinical area of pediatric hematology-oncology. The Phase I training program includes three graduate courses (structure and function of biological molecules, methods for their study, and enzyme kinetics and mechanism), NMR tutorial with the sponsor,and small projects which exploit ongoing research in the sponsor's laboratory and which span the major current areas of NMR. One of two projects will be undertaken during Phase II, with the choice to be determined by feasibility as assessed at the beginning of Phase II. Phase II project A involves a basic study of protein folding, and Phase II project B involves NMR and kinetic studies of a retroviral protease homologous to that from human immunodeficiency virus I. The candidate's long range goal is to use fundamental protein biophysics to contribute strategies for rational drug design.

拟议的医师科学家培训计划将培训现代核磁共振（NMR）方法的候选人，评价大分子的结构和动力学，特别是蛋白质，并将加深他对蛋白质的了解结构-功能关系。候选人稍后将申请这些技术，加上他对酶动力学的了解，研究他所选择的儿科血液肿瘤学临床领域。 I期培训计划包括三个研究生课程（结构和生物分子的功能，研究方法，酶动力学和机制），与申办者一起进行NMR教程，以及利用赞助商正在进行的研究的小项目实验室和跨越NMR的主要当前领域。之一第二阶段将开展两个项目，根据开始时评估的可行性确定第二阶段。第二阶段项目A涉及蛋白质的基础研究第二阶段项目B包括NMR和动力学研究一种逆转录病毒蛋白酶，免疫缺陷病毒I 候选人的长期目标是利用基本的蛋白质生物物理学，合理的药物设计