STRUCTURAL AND DYNAMIC STUDIES OF PROTEINS BY NMR

拟议的医生科学家培训计划将培训 现代核磁共振方法的候选方法 评价大分子的结构和动力学， 尤其是蛋白质，并将加深他对蛋白质的欣赏 结构-功能关系。应聘者稍后将申请 这些技术，加上他对酶动力学的了解， 对他所选择的具有医学意义的分子的研究 儿科血液肿瘤学的临床领域。第一阶段 培训方案包括三门研究生课程(结构和 生物分子的功能、研究方法和 酶动力学和机理)，与赞助商的核磁共振教程，以及 利用赞助商正在进行的研究的小型项目 实验室，它跨越了核磁共振的主要当前领域。其中之一 第二阶段将开展两个项目，可供选择 由#年初评估的可行性决定 第二阶段第二阶段项目A涉及对蛋白质的基础研究 折叠，第二阶段项目B涉及核磁共振和动力学研究 一种与人类同源的逆转录病毒蛋白 免疫缺陷病毒一号候选人的长期目标是 利用基础蛋白质生物物理学为 合理的药物设计。

The proposed Physician Scientist training program will train the candidate in modern nuclear magnetic resonance (NMR) methods for evaluation of structure and dynamics of macromolecules, particularly proteins, and will deepen his appreciation of protein structure-function relationships. The candidate will later apply these techniques, together with his knowledge of enzyme kinetics, to the study of medically significant molecules within his chosen clinical area of pediatric hematology-oncology. The Phase I training program includes three graduate courses (structure and function of biological molecules, methods for their study, and enzyme kinetics and mechanism), NMR tutorial with the sponsor,and small projects which exploit ongoing research in the sponsor's laboratory and which span the major current areas of NMR. One of two projects will be undertaken during Phase II, with the choice to be determined by feasibility as assessed at the beginning of Phase II. Phase II project A involves a basic study of protein folding, and Phase II project B involves NMR and kinetic studies of a retroviral protease homologous to that from human immunodeficiency virus I. The candidate's long range goal is to use fundamental protein biophysics to contribute strategies for rational drug design.