MECHANISM AND MODULATION OF OROFACIAL PAIN

这个项目的目的是更好地了解机制和 调节与口腔组织相关的疼痛和 邻近的结构(即牙齿、牙床、面颊、颌肌和 颞下颌关节)。疼痛的机制和痛觉调制 在很大程度上仍不清楚来自口腔的原因。因此，从长远来看， 该项目的目标是改善牙科相关疼痛的管理。 组织损伤会在口腔或周围诱发 结构。这将通过去除牙釉质和牙本质来完成 牙齿，或通过使用各种制剂中的一种来诱导牙齿发炎 牙床、骨/关节、咬肌或检查粘膜。跟随 组织损伤电生理学和行为学研究将 已执行。要使用的刺激将包括电、热、 对牙齿、牙床、牙龈的化学和机械刺激， 咬肌和颞下颌关节。神经元对这些物质的反应 刺激将通过脑内电刺激或 毒品。 清醒大鼠对各种机械和机械刺激的行为反应 化学刺激将在行为分析中进行监测，以及 被动回避范式。在任何情况下，刺激都不会是 不受控制的或产生组织损伤的持续时间的。这些将是 用于确定所描述的 化学和机械刺激与疼痛的病因学有关 发自口腔的。 该项目建议使用多学科方法，包括 各种解剖学、药理学、神经化学和行为学 解决三个具体目标的技巧：(1)检查和比较 行为症状表现的机制 由各种组织侮辱产生；(2)表征 在每种组织损伤中，都有必要的机械或化学刺激 模型，以产生疼痛的行为证据；(3)检查角色 中枢和外周的神经递质和神经调质 神经系统在行为发育和/或维持中的作用 口腔疼痛模型中产生的症状。 这项研究代表了对病理学的系统检查。 口腔致病机制的神经化学和解剖学基础 应提供有关治疗和治疗的有价值的信息 口腔疼痛的管理。 关键词：药理学、神经化学、口腔疼痛

The aims of this project are to better understand the mechanisms and modulation of pain associated with tissue in the oral cavity and adjacent structures (i.e., the teeth, gums, cheek, jaw muscles, and temporomandibular joint). The mechanisms of pain, and pain modulation from the oral cavity remain largely unknown. Therefore, the long term goal of this project is to improve management of dental-related pain. Tissue damage will be induced in the oral the cavity or surrounding structures. This will be done by removing enamel and dentin from the teeth, or by using one of a variety of agents to induce inflammation in the gums, bone/joints, masseter muscle, or check mucosa. Following tissue insult electrophysiology and behavioral studies will be performed. Stimuli to be used will include electrical, thermal, chemical, and mechanism stimulation of the teeth, gums, gingiva, masseter, and temporomandibular joint. Neuronal responses to these stimuli will be modulated by intracerebral electrical stimulation or drugs. In conscious rats behavioral responses to various mechanical and chemical stimuli will be monitored in behavioral assays as well as a passive avoidance paradigm. In no cases will the stimulus be uncontrolled or of duration that produces tissue damage. These will be the definitive experiments used in determining whether the described chemical and mechanical stimuli are involved in the etiology of pain arising from the oral cavity. This project proposes to use a multi-disciplinary approach incorporating a variety of anatomical, pharmacological, neurochemical and behavioral techniques to address three specific aims: (1) to examine and compare the mechanisms responsible for the expression of the behavioral symptoms produced by the various tissue insults; (2) to characterize the necessary stimulus, either mechanical or chemical, in each tissue insult model, to produce behavioral evidence of pain; (3) to examine the role of neurotransmitters and neuromodulators in the central and peripheral nervous systems in the development and /or maintenance of the behavioral symptoms produced in oral cavity pain models. The research represents a systematic examination of the pathology neurochemistry, and anatomy underlying the causative mechanisms of oral pain and should yield valuable information concerning the treatment and management of oral pain. KEYWORDS: Pharmacological, Neurochemical, Oral Pain