CYTOKINES IN CHRONIC ADULT PERIDONTITIS

成人牙周炎是一种慢性炎症性疾病， 牙齿的支撑结构。 毒力之间的相互作用 菌斑中致病微生物与宿主免疫的关系 反应是疾病的主要诱因。 许多炎性细胞因子 与疾病过程有关，尽管直接证据是 目前有限。 革兰氏阴性杆菌的脂多糖（LPS） 细菌可以调节牙周局部的炎症反应 牙周袋和相关的慢性性质 感染，连接组织的逐渐破坏，以及 牙槽骨 由于龈沟液中含有细胞分泌的产物 在牙龈中，我们开发了确定其存在和水平的方法 几种炎性和抗炎性细胞因子 收集自临床健康和牙周病部位。 通过 蛋白质印迹法检测肿瘤坏死细胞因子蛋白 因子-α（TNF-α）、白细胞介素-6（IL-6）和转化生长 在80 ng、40 ng和10 ng的敏感性水平下的β因子（TGF-β）， 分别 我们的研究涉及收集发炎的 牙周组织和细胞因子基因表达的检查包括 细胞因子信使RNA的反向扩增 转录酶-聚合酶链反应。 我们发现了 细胞因子IL-1 α、1 - 1 β、TNF-α、IL-6和IL-8的信息 在这些组织中。 单个核细胞对各种LPS的体外反应的检查 然后进行制备，培养单核细胞系， U937与不同量的来自卟啉单胞菌或 大肠杆菌与增殖、细胞活力和炎性 评估细胞因子基因表达。 我们的研究结果表明， 两种效应的浓度和时间依赖性差异 细胞上的LPS制剂。

Adult periodontitis is a chronic inflammatory disease involving the supporting structures of the teeth. The interaction between virulence factors of microbial pathogens in dental plaque and the host immune response is a major inducer of the disease. Many inflammatory cytokines have been implicated in the disease process, although direct evidence is presently limited. The lipopolysaccharide (LPS) from gram-negative bacteria may regulate the local inflammatory response in the periodontal pocket and be associated with the chronic nature of the periodontal infection, the gradual destruction of connection tissue, and loss of alveolar bone. Since the gingival crevicular fluid contains products secreted by cells in the gingiva, we developed methods to determine the presence and level of several inflammatory and anti-inflammatory cytokins in samples collected from clinically health and periodontally diseased sites. By Western blot we detected the cytokine proteins tumor necrosis factor-alpha (TNF-alpha), interleukin-6 (IL-6), and transforming growth factor-beta (TGF-beta) at sensitivity levels of 80 ng, 40 ng, and 10 ng, respectively. Our studies involving the collection of inflamed periodontal tissues and examination of cytokine gene expression included amplification of cytokine messenger RNA by the reverse transcriptase-polymerase chain reaction. We identified the presence of the message for cytokines IL-1alpha, l-1beta, TNF-alpha, IL-6, and IL-8 in these tissues. Examination of the in vitro response of mononuclear cells to various LPS preparations was then undertaken, Cultures of the monocytic cell line, U937, were incubated with various amounts of LPS from Porphyromonas or Escherichia coli and proliferation, cell viability, and inflammatory cytokine gene expression were assessed. Our results indicate both concentration and time dependent differences in the effects of the two LPS preparations on the cells.