CELLULAR MECHANISMS OF NEUROENDOCRINE HORMONE SECRETION

描述：(改编自申请者摘要)长期 目的是了解神经对激素分泌的控制及其 海洋软体动物对生殖生理和行为的影响 卡氏海兔作为动物模型。因为他们单纯的紧张 系统和大型神经元，海兔已被广泛用于生物物理， 神经内分泌细胞的细胞和分子研究(称为 袋细胞)控制排卵和产卵行为。然而， 关于调节荷尔蒙的过程的研究还很少。 这些细胞的分泌物。拟议的工作将使用袋子电池和 它们分泌的产卵激素(ELH)来鉴定细胞 调节激素分泌动态变化的机制。这个 具体目的是确定：袋子细胞的电信号模式是否 体内和体内的后放电和ELH分泌相似 体外制剂；并确定二酰甘油的作用 (DAG)、钙和cAMP在调节ELH分泌中的作用。

DESCRIPTION: (adapted from Applicant's Abstract) The long-term objective is to understand neural control of hormone secretion and its impact on reproductive physiology and behavior using the marine mollusk Aplysia californica as an animal model. Because of their simple nervous system and large neurons, Aplysia have been widely used for biophysical, cellular and molecular investigations of the neuroendocrine cells (called bag cells) that control ovulation and egg-laying behavior. However, there has been little work on the processes that regulate hormone secretion from these cells. The proposed work will use bag cells and their secretion of egg-laying hormone (ELH) to identify cellular mechanisms that regulate dynamic changes in hormone secretion. The specific aims are to determine: whether patterns of bag-cell electrical afterdischarge and ELH secretion are similar between in vivo and in vitro preparations; and to determine the role of the diacylglycerol (DAG), calcium and cAMP in regulating ELH secretion.