CELLULAR MECHANISMS OF NEUROENDOCRINE HORMONE SECRETION

神经内分泌激素分泌的细胞机制

• 批准号: 2272397
• 负责人: NANCY L WAYNE
• 金额: $ 15.57万
• 依托单位: UNIVERSITY OF CALIFORNIA LOS ANGELES
• 依托单位国家: 美国
• 财政年份: 1994
• 资助国家: 美国
• 起止时间: 1994-12-01 至 1998-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2272397
• 关键词: Aplysia; aminopyridines; calcium; calcium channel blockers; chelating agents; cyclic AMP; diacylglycerols; ganglions; hormone regulation /control mechanism; membrane potentials; neuroendocrine system; neuropharmacology; protein kinase C; reproductive hormone; secretion

DESCRIPTION: (adapted from Applicant's Abstract) The long-term objective is to understand neural control of hormone secretion and its impact on reproductive physiology and behavior using the marine mollusk Aplysia californica as an animal model. Because of their simple nervous system and large neurons, Aplysia have been widely used for biophysical, cellular and molecular investigations of the neuroendocrine cells (called bag cells) that control ovulation and egg-laying behavior. However, there has been little work on the processes that regulate hormone secretion from these cells. The proposed work will use bag cells and their secretion of egg-laying hormone (ELH) to identify cellular mechanisms that regulate dynamic changes in hormone secretion. The specific aims are to determine: whether patterns of bag-cell electrical afterdischarge and ELH secretion are similar between in vivo and in vitro preparations; and to determine the role of the diacylglycerol (DAG), calcium and cAMP in regulating ELH secretion.

描述：（改编自申请人的摘要） 目的是了解激素分泌的神经控制及其 利用海洋软体动物对生殖生理和行为的影响 以加州紫茎泽兰为动物模型。 因为他们单纯的神经质 系统和大型神经元，失智症已被广泛用于生物物理， 神经内分泌细胞的细胞和分子研究（称为 囊细胞），控制排卵和产卵行为。 然而，在这方面， 关于调节激素的过程， 这些细胞的分泌物。 拟议的工作将使用袋细胞和 其分泌的产卵激素（ELH），以确定细胞 调节激素分泌动态变化的机制。 的 具体目标是确定：袋式电池的电模式是否 后放电和ELH分泌在体内和体内是相似的， 体外制剂;并确定甘油二酯的作用 (DAG)Ca ~（2+）和cAMP对ELH分泌的调节作用。