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PHOSPHOLAMBAN AND HYPOTHYROID AND HYPERTHYROID HEARTS

磷光班与甲状腺功能减退症和甲状腺功能亢进症心脏

基本信息

批准号：
2292986
负责人：
EVA KISS
金额：
$ 3.48万
依托单位：
UNIVERSITY OF CINCINNATI
依托单位国家：
美国
项目类别：
财政年份：
1994
资助国家：
美国
起止时间：
1994-06-01 至
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/2292986
关键词：
PHOSPHOLAMBAN HYPOTHYROID HYPERTHYROID HEARTS

项目摘要

My research goal under the Fogarty Fellowship would be to clarify the regulatory role of phospholamban on the SR Ca2+-pump in various thyroid states (hypothyroid, euthyroid and hyperthyroid). These studies will provide an appropriate approach to the determination of the role of phospholamban in regulation of the SR function in thyroid hearts and its involvement in mediating the altered response of these hearts to Beta- adrenergic stimulation. Such studies may provide the basis for an approach to the treatment of thyroid disease with more rational experiments. At Albert Szent-Gyorgyi Medical University in Hungary I work in cooperation with a clinical cardiologist and plan to start biochemical studies on cardiomyopathic heart muscle. Accordingly, the various biochemical techniques with which the SR function can be studied under in vitro and in vivo conditions (SR preparation, characterization, purificatioin of phospholamban, reconstitution of SR membrane vesicles, measurement of Ca2+ uptake and Ca2+-ATPase activity, radioimmunoassay techniques, heart perfusion, etc.) would be extremely important for me. At Prof. Kranias's laboratory in Cincinnati, I have a very good chance to master all of these methods, since all of the necessary equipment is available and good experts can help me to become familiar with all of the relevant techniques.

我在福格蒂奖学金下的研究目标是澄清受磷蛋白对不同甲状腺组织SR钙泵的调节作用状态（甲状腺功能减退、甲状腺功能正常和甲状腺功能亢进）。这些研究将提供一个适当的方法来确定的作用受磷蛋白对甲状腺心脏SR功能的调节作用及其机制参与介导这些心脏对β-受体的反应改变，肾上腺素能刺激这些研究可以为一个甲状腺疾病的治疗方法更合理实验在匈牙利的阿尔伯特圣捷尔吉医科大学，与临床心脏病专家合作，并计划开始生化心肌病的研究。因此，各种生物化学技术，与SR功能可以研究下，体外和体内条件（SR制备，表征，受磷蛋白的纯化，SR膜囊泡的重建， Ca 2+摄取和Ca 2 +-ATP酶活性测定，放射免疫法技术、心脏灌注等）对我来说非常重要在辛辛那提的克拉尼亚斯教授的实验室里，我很有可能掌握所有这些方法，因为所有必要的设备都是可用的和好的专家可以帮助我熟悉所有的相关技术。