PROPERTIES OF AMPLIFIED GENES FOR RESISTANCE IN CULEX

库蚊扩增抗性基因的特性

基本信息

  • 批准号:
    2071668
  • 负责人:
  • 金额:
    $ 6.18万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
  • 财政年份:
    1994
  • 资助国家:
    美国
  • 起止时间:
    1994-07-15 至 1998-07-14
  • 项目状态:
    已结题

项目摘要

Insecticide resistance is a serious obstacle to the effective control of insect vectors of disease. My research program is directed toward understanding the genetic and population processes influencing the evolution of insecticide resistance in vector populations. The long-term goal of my work is to provide the information necessary to design vector control strategies which minimize the rate of evolution of resistance. In the mosquito Culex quinquefasciatus, resistance to organophosphate insecticides is associated with a highly active esterase allozyme, designated EST-B1. The molecular basis of high esterase activity in resistant individuals is amplification (increase in copy number) of the EST-B1 gene. To understand the dynamics of amplified EST-B1 genes in populations we need information about how genetic processes interact with insecticidal selection to produce individuals with high esterase activity, the stability properties of these high activity phenotypes, and how esterase activity phenotype affects reproductive fitness in the presence of absence of insecticide. The specific objectives of the proposed research are: 1) A chromosome carrying and EST-B1 gene amplification which results in a small bud readily detectable increase in esterase activity over susceptible strain levels will be isolated from the S-Y strain of C quinquefasciatus which exhibits a wide range of esterase activity phenotypes. Strains carrying this chromosome will be subjected to insecticidal selection to assess changes in the frequency and distribution of esterase activity phenotypes over time. This experiment will provide a rough estimate of the frequency with which esterase activity variants arise, and how quickly high esterase activity phenotypes are produced by selection. 2) The stability of high esterase activity phenotypes produced by selection will be determined by examining changes in the frequency and distribution of esterase activity phenotypes over time in strains released from insecticidal selection. 3) The relationship between esterase activity phenotype and the ability to survive insecticide treatment will be determined in the variable S-Y strain by subjecting samples of Individuals to different concentrations of insecticide and measuring the esterase activity of survivors. 4) The relationship between esterase activity phenotype and reproductive fitness will be determined in the variable S-Y strain by measuring esterase activity and several components of reproductive fitness of individuals.
杀虫剂抗药性是有效控制该虫的一个严重障碍。 昆虫传播疾病的媒介。我的研究计划针对的是 了解影响遗传和种群过程的 媒介种群对杀虫剂抗性的进化。长期的 我的工作目标是为设计载体提供必要的信息 将抗药性进化速度降至最低的控制策略。 致倦库蚊对有机磷的抗性 杀虫剂与一种高活性的酯酶等位酶有关, 编号为EST-B1。大豆酯酶活性较高的分子基础 抗性个体是基因的扩增(拷贝数增加) Est-B1基因。为了了解扩增出的EST-B1基因的动态变化 我们需要关于遗传过程如何与之相互作用的信息 杀虫选择,以产生高酯酶活性的个体, 这些高活性表型的稳定性特性,以及如何 酯酶活性表型影响生殖适合性 没有杀虫剂。 拟议研究的具体目标是:1)一条染色体 携带和EST-B1基因扩增,导致小芽 与敏感品系相比,酯酶活性明显增加 表达水平将从致倦库蚊S-Y株中分离出来 表现出广泛的酯酶活性表型。携带的菌株 这条染色体将接受杀虫选择,以评估 酯酶活性表型频率和分布的变化 随着时间的推移。这个实验将提供一个粗略的频率估计。 随着酯酶活性变异的出现,以及高酯酶的速度有多快 活动表型是通过选择产生的。2)高的稳定性 通过选择产生的酯酶活性表型将由 检查酯酶活性的频率和分布的变化 从杀虫选择释放的菌株的表型随时间的变化。3) 酯酶活性表型与细胞免疫功能的关系 成活杀虫剂处理后将测定变量S-Y 通过将个体样本置于不同浓度的 杀虫剂和测定幸存者的酯酶活性。4) 酯酶活性表型与生殖适合度的关系 将在S-Y变异株中通过测定酯酶来确定 活动和个体生殖适宜性的几个组成部分。

项目成果

期刊论文数量(1)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)

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