Protein Structural-Dynamics at Solid Surfaces: Beyond Static Snapshots

固体表面的蛋白质结构动力学：超越静态快照

• 批准号: FT190100451
• 负责人: Prof Michael Higgins
• 金额: $ 69.51万
• 依托单位: University of Wollongong
• 依托单位国家: 澳大利亚
• 项目类别: ARC Future Fellowships
• 财政年份: 2020
• 资助国家: 澳大利亚
• 起止时间: 2020-05-20 至 2024-05-19
• 项目状态: 已结题
• 来源: https://dataportal.arc.gov.au/RGS/Web/Grants/FT190100451
• 关键词: Protein; Structural; Dynamics; Solid; Surfaces

The project will use High-Speed Atomic Force Microscopy to directly visualize single proteins in ‘action’ with surfaces, revealing their dynamics at unprecedented combined structural and temporal resolution in liquid. Such characterization moves beyond static ‘snapshots’ of protein structure, toward the dynamic changes in protein conformation that will enable new exploration of key biological processes at liquid-solid interfaces. New fundamental discoveries will have an impact on technologies such as medical device coatings, biomaterials, biosensors, microfluidics devices, protein purification and diagnostics assays that are critically dependent on the biological function of adsorbed or immobilized proteins.

该项目将使用高速原子力显微镜直接可视化与表面“作用”的单个蛋白质，以前所未有的结构和时间分辨率揭示它们在液体中的动力学。这种表征超越了蛋白质结构的静态“快照”，走向蛋白质构象的动态变化，这将使新的探索在液-固界面的关键生物过程。新的基础发现将对医疗器械涂层、生物材料、生物传感器、微流体设备、蛋白质纯化和诊断分析等技术产生影响，这些技术严重依赖于吸附或固定蛋白质的生物功能。