CHRONIC AND ACUTE CYTOTOXICITY IN KIDNEY CELLS

肾细胞的慢性和急性细胞毒性

• 批准号: 2133781
• 负责人: LAWRENCE H. LASH
• 金额: $ 6.67万
• 依托单位: WAYNE STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1993
• 资助国家: 美国
• 起止时间: 1993-09-01 至 1998-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2133781
• 关键词: adenine nucleotides; chronic disease /disorder; cytotoxicity; density gradient ultracentrifugation; glutathione; high performance liquid chromatography; histochemistry /cytochemistry; immunofluorescence technique; kidney cell; laboratory rat; oxygen consumption; phase contrast microscopy; renal toxin; renal tubule; scanning electron microscopy; tissue /cell culture; toxin metabolism; transmission electron microscopy

The candidate, Dr. Lawrence Lash, is an Assistant Professor in the Department of Pharmacology at Wayne State University School of Medicine. Dr. Lash has established an active research program on biochemical mechanisms of nephrotoxicity. His research is currently supported by a FIRST Award from NIH (1988-1993) and he was the recipient of a Pharmaceutical Manufacturers Foundation Association Research Starter Award (1988-1989). During Dr. Lash's research career he has made significant contributions toward our understanding of the roles of renal glutathione in physiological and toxicological processes and of the mechanistic basis of chemical-induced nephrotoxicity. He has established two research collaborations which ave resulted in applications of new techniques in his research program. In the four years since joining the faculty, Dr. Lash has become very active in departmental programs and activities. Dr. Lash's immediate career goals are to continue developing his research program and his professional collaborations and thereby establish himself further as a recognized researcher in the area of biochemical toxicology. In preliminary studies, Dr. Lash's laboratory has established primary cultures of renal proximal tubular cells as an in vitro model system to study chronic toxicity and carcinogenesis. This new methodology provides opportunities for expansion and an increased sophistication in the research program. His long-term career goals are to maintain an active research program, learn new techniques, foster new research collaborations, become more active in the scientific community, and become more involved in the training of students and postdoctoral fellows. Dr. Lash is one of only two Assistant Professors and one of only three non-tenured faculty members in a department of eighteen full- time faculty. Receipt of a Research Career Development Award will provide more job security but more importantly, will allow the candidate to spend more time in development of his research program, in fostering collaborations, and in learning new techniques and research approaches. If given this award, the Department of Pharmacology will support the research development of the candidate by requiring less participation in departmental committees and teaching, reducing non-research obligations to 10 to 15% of effort, and by allowing the candidate to spend brief periods of time (one to four months) at other laboratories learning new methodology. The research that will be performed during the tenure of this proposed award will involve a continuation of studies in freshly isolated renal cells on the biochemical basis of cellular susceptibility to various forms of acute injury produced by toxic chemicals and pathological conditions and new studies on development of primary cultures of renal cells from specific nephron regions and their use in the study of biochemical mechanisms of chronic toxicity.

候选人Lawrence Lash博士是该校的助理教授 韦恩州立大学医学院药理学系。 拉什博士建立了一个积极的研究项目， 肾毒性机制。 他的研究目前得到了一个 第一个奖从美国国立卫生研究院（1988年至1993年），他是一个收件人 制药商基金会协会研究启动者 1988 - 1989年）。 在拉什博士的研究生涯中， 对我们理解肾脏的作用有重大贡献， 谷胱甘肽在生理和毒理过程中的作用以及 化学性肾毒性的机制基础。 他建立 两项研究合作，导致了新的 他的研究计划中的技术。 在加入后的四年里， 教师，博士拉什已成为非常活跃的部门计划， 活动 拉什博士的近期职业目标是继续发展 他的研究项目和他的专业合作， 进一步确立了自己作为一个公认的研究领域， 生化毒理学 在初步研究中，拉什博士的实验室 已经建立了肾近端小管细胞的原代培养， 研究慢性毒性和致癌作用体外模型系统。 这 新的方法提供了扩展的机会， 研究计划的复杂性。 他的长期职业目标是 保持积极的研究计划，学习新技术，培养新的 研究合作，在科学界变得更加活跃， 并更多地参与学生和博士后的培训 伙计们 拉什博士是仅有的两个助理教授之一， 只有三个非终身教职员工在一个部门的十八个完整的， 时间教师 获得研究职业发展奖将 提供更多的工作保障，但更重要的是， 花更多的时间发展他的研究计划， 合作，学习新技术和研究方法。 如果获得这个奖项，药理学系将支持 通过要求较少的参与来促进候选人的研究发展 部门委员会和教学，减少非研究义务 10%到15%的努力，并允许候选人花简短的时间， 在其他实验室学习新知识的时间（1至4个月） 方法论 将在任期内进行的研究 这项拟议的奖励将涉及在新鲜的研究继续 细胞易感性生化基础上的分离肾细胞 有毒化学品造成的各种形式的急性损伤， 原发性肝癌发生的病理条件和新的研究 来自特定肾单位区域的肾细胞的培养物及其在 研究慢性毒性的生化机制。