MICROTUBULE DYNAMICS IN OOCYTES AND ZYGOTE

卵母细胞和受精卵中的微管动力学

• 批准号: 2293260
• 负责人: PETER Sutovsky SUTOVSKY
• 金额: $ 2.5万
• 依托单位: UNIVERSITY OF WISCONSIN-MADISON
• 依托单位国家: 美国
• 财政年份: 1994
• 资助国家: 美国
• 起止时间: 1994-09-30 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/2293260
• 关键词: MICROTUBULE; DYNAMICS; OOCYTES; ZYGOTE

The proposed research is aimed to contribute the data on the role of microtubules in human oogenesis and fertilization, using Rhesus monkey as a model animal. Four basic questions will be addressed: 1. What is the organization of microtubules and meiotic apparatus during oocyte maturation and fertilization? 2 Is there evidence for a paternal centrosome contribution during normal fertilization and polyspermy? 3. Will parthenogenetically activated Rhesus oocytes organize a bipolar mitotic apparatus at the time of first division? 4. What is the role of microtubules during Rhesus fertilization? The methods used in the study include conventional immunofluorescence, laser scanning confocal microscopy, transmission electron microscopy, time lapse video microscopy, ultrastructural immunocytochemistry and the methods of in vitro maturation and fertilization. The immunocytochemical methods will take profit from the availability of new antibodies against alpha, beta - tubulins, kinetochores, centrosomes and microtubule organizing centers. The expected results should accomplish the knowledge about basic processes occurring during the oogenesis and fertilization, which belong to the most important problems of human reproduction.

拟议的研究旨在提供有关作用的数据 使用恒河猴研究微管在人类卵子发生和受精中的作用 作为模型动物。 将解决四个基本问题： 1. 什么是 卵母细胞期间微管和减数分裂器的组织 成熟和受精？ 2 有没有父子关系的证据 正常受精和多精受精过程中中心体的贡献？ 3. 孤雌生殖激活的恒河猴卵母细胞会组织双相吗 第一次分裂时有丝分裂装置？ 4. 作用是什么 恒河猴受精过程中微管的变化？ 中使用的方法 研究包括常规免疫荧光、激光扫描共焦 显微镜、透射电子显微镜、延时视频 显微镜、超微结构免疫细胞化学及其方法 体外成熟和受精。 免疫细胞化学方法将 从针对 α、β 的新抗体的可用性中获利 - 微管蛋白、动粒、中心体和微管组织中心。 预期的结果应该完成基本知识 发生在卵子发生和受精过程中的过程，属于 人类繁衍最重要的问题。