MICROTUBULE DYNAMICS IN OOCYTES AND ZYGOTE

拟议的研究旨在提供有关以下方面作用的数据： 微管在人类卵子发生和受精中的作用 作为一个模型动物。 四个基本问题将得到解决：1。 是什么 卵母细胞中微管和减数分裂器组织 成熟和受精 2、有证据表明， 在正常受精和多精受精过程中中心体的贡献？ 3. 孤雌激活的恒河猴卵母细胞是否会形成双极 第一次分裂时的有丝分裂器 4. 有什么作用 在恒河猴受精过程中微管的变化 中所使用的方法 研究包括常规免疫荧光、激光扫描共聚焦 显微镜，透射电子显微镜，时间推移视频 显微镜、超微结构免疫细胞化学及免疫组化方法 体外成熟和受精。 免疫细胞化学方法将 利用新的抗体对抗α，β- 微管蛋白、动粒、中心体和微管组织中心。 预期的结果应该完成的基本知识 在卵子发生和受精过程中发生的过程，属于 人类生殖最重要的问题。

The proposed research is aimed to contribute the data on the role of microtubules in human oogenesis and fertilization, using Rhesus monkey as a model animal. Four basic questions will be addressed: 1. What is the organization of microtubules and meiotic apparatus during oocyte maturation and fertilization? 2 Is there evidence for a paternal centrosome contribution during normal fertilization and polyspermy? 3. Will parthenogenetically activated Rhesus oocytes organize a bipolar mitotic apparatus at the time of first division? 4. What is the role of microtubules during Rhesus fertilization? The methods used in the study include conventional immunofluorescence, laser scanning confocal microscopy, transmission electron microscopy, time lapse video microscopy, ultrastructural immunocytochemistry and the methods of in vitro maturation and fertilization. The immunocytochemical methods will take profit from the availability of new antibodies against alpha, beta - tubulins, kinetochores, centrosomes and microtubule organizing centers. The expected results should accomplish the knowledge about basic processes occurring during the oogenesis and fertilization, which belong to the most important problems of human reproduction.