MICROTUBULE DYNAMICS IN OOCYTES AND ZYGOTE

这项拟议的研究旨在为以下方面提供数据： 恒河猴卵子发生和受精过程中的微管 作为模范动物。将解决四个基本问题：1.什么是 卵母细胞中微管和减数分裂器官的组织 成熟和受精？2有证据表明 中心体在正常受精和多精受精过程中的贡献？ 孤雌生殖激活的恒河猴卵母细胞是否会形成两极 第一次分裂时的有丝分裂机构？4.作用是什么 恒河猴受精过程中微管的数量？中使用的方法 研究包括常规免疫荧光、激光扫描共聚焦 显微镜、透射电子显微镜、时间推移视频 显微镜、超微结构免疫细胞化学及免疫组织化学方法 体外成熟和受精。免疫细胞化学方法将 从新的抗α，β-抗体中获利- 微管蛋白、动粒、中心体和微管组织中心。 预期结果应具备以下基本知识 在卵子发生和受精期间发生的过程，属于 人类繁衍的最重要的问题。

The proposed research is aimed to contribute the data on the role of microtubules in human oogenesis and fertilization, using Rhesus monkey as a model animal. Four basic questions will be addressed: 1. What is the organization of microtubules and meiotic apparatus during oocyte maturation and fertilization? 2 Is there evidence for a paternal centrosome contribution during normal fertilization and polyspermy? 3. Will parthenogenetically activated Rhesus oocytes organize a bipolar mitotic apparatus at the time of first division? 4. What is the role of microtubules during Rhesus fertilization? The methods used in the study include conventional immunofluorescence, laser scanning confocal microscopy, transmission electron microscopy, time lapse video microscopy, ultrastructural immunocytochemistry and the methods of in vitro maturation and fertilization. The immunocytochemical methods will take profit from the availability of new antibodies against alpha, beta - tubulins, kinetochores, centrosomes and microtubule organizing centers. The expected results should accomplish the knowledge about basic processes occurring during the oogenesis and fertilization, which belong to the most important problems of human reproduction.