FERAL MOUSE BREEDING COLONY

野鼠繁殖群

• 批准号: 3700956
• 负责人: EVELYN HOGG
• 金额: $ 13.98万
• 依托单位: BIOTECHNOLOGY RESEARCH INSTITUTE
• 依托单位国家: 美国
• 财政年份: 1994
• 资助国家: 美国
• 起止时间: 1994-12-01 至 1998-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3700956
• 关键词: animal colony; biomedical facility; genetically modified animals; laboratory mouse

Goal: Induction of mammary tumors with biological (hormones, retroviral shuttle vectors, and mouse mammary tumor virus, MMTV) and chemical carcinogens in various feral strains of Mus musculus and other species of Mus. Breeding of transgenic strains of Mus musculus containing certain activated protooncogenes. Maintenance of preneoplastic mammary hyperplastic outgrowth lines by transplantation in syngeneic mice. Approach: Maintain a closed pedigreed colony of 1,000 feral and inbred mice. The colony is composed of approximately 700 mice that are held long term (2 years) for tumor development and 300 mice as a breeding nucleus. The breeding nucleus is composed of three pedigreed outbred colonies of feral mice having unique characteristics that are pertinent to the study of mouse mammary tumorigenesis. They are: CZECHII V-mice (Mus musculus musculus), CZECHII V+, MS (M. spretus) mice. Five transgenic mouse lines containing the MMTVLTR-Int3, WAP-Int3, MMTVLTR-Wnt-1, MMTVLTR-TGFb, and MT-TGFa transgenes are also being maintained. The TGFa "knockout" mouse strain has also recently been introduced into the colony. In addition, a limited breeding nucleus of the high-incidence C3H/OuJ, GR/imr, BALB/cfCZECHII, and BALB/cfMS inbred mouse strains and the low-incidence BALB/cp and FVB inbred mouse strains are maintained.

目标：用生物(激素、逆转录病毒)诱导乳腺肿瘤 穿梭载体和小鼠乳腺肿瘤病毒(MMTV)和化学品 不同野生品系的小鼠和其他物种的致癌物质 Mus.含特定成分转基因小鼠品系的选育 激活的原癌基因。癌前乳房的维护 移植于同基因小鼠的增生性突起系。 方法：维持一个由1,000头野生和近亲繁殖的封闭血统群体 老鼠。这个群体由大约700只长期持有的小鼠组成。 以300只小鼠为繁育核心的肿瘤发展期(2年)。 繁育核心由三个纯种近交群体组成。 具有与研究相关的独特特征的野生小鼠 关于小鼠乳腺肿瘤发生的研究。它们是：CZECHII V-小鼠(小鼠 Musculus)、CZECHII V+、MS(M.spretus)小鼠。五个转基因小鼠品系 包含MMTVLtr-Int3、WAP-Int3、MMTVLtr-WNT-1、MMTVLtr-TGFb和 MT-TGFa转基因也得到了维持。TGFa“基因敲除”小鼠 菌株最近也被引入了这个殖民地。此外，a 高发C3H/OuJ、GR/IMR、 BALB/cfCZECHII、BALB/cfMS近交系小鼠及其低发率 BALB/cp和FVB近交系小鼠保持不变。