BIOCHEMICAL GENETIC MONITORING IN RODENTS

承包商应：通过电泳、红细胞裂解物， 和组织匀浆，利用七（7）和十二（12）之间的 每个品系的指定位点;接收十（10）只近交系或杂交小鼠 每周从两（2）个菌株/杂交种中的每一个菌株/杂交种中分离;并且能够 检测由所列每个基因座编码的蛋白质或酶 下面通过电泳： 基因座 染色体 描述 IDH-1 1 异柠檬酸脱氢酶 PEP-3 1 二肽酶 汽车-2 3 红细胞碳酸酐酶 GPD-1 4 葡萄糖-6-磷酸脱氢酶 Pgm-1 5 变位酶 Ldr-1 6 乳酸脱氢酶调节剂 Gpi-1 7 葡萄糖磷酸异构酶 HBB 7 血红蛋白β链 Es-1 8 酯酶 Mod-1 9 苹果酸酶 Es-3 11 酯酶 Es-10 14 酯酶 能够监测以下菌株/杂交种。 项目 如有必要，官员可添加其他菌株/杂交菌株。 A/J NFS/NCr AKR/N RFM/UN BALB/Cann NZB C57BL/6N NZW C57BL/10ScN B10.A

The contractor shall: monitor by electrophoresis, erythrocytelysates, and tissue homogenates utilizing between seven (7) and twelve (12) designated loci for each strain; receive ten (10) inbred or hybrid mice per week from each of two (2) strains/hybrids; and be capable of detecting the proteins or enzymes encoded by each of the loci listed below by means of electrophoresis: Locus Chromosome Description Idh-1 1 isocitrate dehydrogenase Pep-3 1 dipeptidase Car-2 3 RBC carbonic anhydrase Gpd-1 4 glucose-6-phosphate dehydrogenase Pgm-1 5 phosphoglucomutase Ldr-1 6 lactate dehydrogenase regulator Gpi-1 7 glucose phosphate isomerase Hbb 7 hemoglobin-beta chain Es-1 8 esterase Mod-1 9 malic enzyme Es-3 11 esterase Es-10 14 esterase Be capable of monitoring the following strains/hybrids. The Project Officer may add other strains/hybrids if necessary. A/J NFS/NCr AKR/N RFM/UN BALB/cAnN NZB C57BL/6N NZW C57BL/10ScN B10.A