EXPRESSION--SYNTHETIC DNA SEQUENCES/HTLV-I ENV PROTEN

人I细胞白血病病毒I（HTLV-I）是一种转化型逆转录病毒 病因与成人T细胞白血病/淋巴瘤相关， 热带痉挛性麻痹。 HIV-1血清阳性人数 美国的献血者人数正变得惊人地高， 关于可回收血液制品的污染 发展 一种高灵敏度和高特异性的血液筛查方法， 血清中的HTLV-I抗体受到数量限制的阻碍 包括免疫原性最强的蛋白质， 信封. 拟议的第一阶段研究概述了一项战略， 表达选定的部分以及整个信封 蛋白质使用合成的DNA编码序列。 预计 这种方法可以让这些多肽 在大肠杆菌中的高水平。 所表达的多肽将是 进行生化分析，并评估其检测能力， 人血清中HTL 7-I的抗体。 在第二阶段， 候选包膜多肽将被用于配置 酶联免疫测定（EIA）检测HTLV-I抗体， 人血清 环境影响评估将进行敏感性评估， 使用来自大量正常和感染的血清的特异性 个体 据设想， 重组衍生的HTL 7-I蛋白将被配置成高- 高质量的血液筛查检测是否接触过这种病毒

Human I-cell leukemia virus I (HTLV-I) is a transforming retrovirus etiologically associated with adult T-cell leukemia/lymphoma and troPical spastic ParaParesis. The number of HILV-I seropositive blood donors in the US is becoming alarmingly high raising concerns about contamination of transfusable blood products. Development of a highly-sensitive and highlyspecific blood-screening assay for HTLV-I antibodies in serum has been hindered by limiting quantities of certain viral proteins, including the most immunogenic protein, envelope. The proposed Phase I study outlines a strategy for expressing selected portions, as well as the entire envelope protein using synthetic DNA coding sequences. It is anticipated that this approach will allow these polypeptides to be expressed at high levels in E.coli. The expressed polypeptides will be analyzed biochemically and evaluated for their ability to detect antibodies to HTL7-I in human sera. In Phase II, promising candidate envelope polypeptides will be used to configure an enzyme-linked immunoassay (EIA) to detect antibodies to HTLV-I in human sera. The EIA will be evaluated for sensitivity and specificity using sera from a large number of normal and infected individuals. It is envisaged that a complementary set of recombinant-derived HTL7-I proteins will be configured into a high- quality blood-screening assay for exposure to this virus.