SEX STEROID AND V VULNIFICUS ENDOTOXIC SCHOCK

描述(改编自《调查者摘要》)：这是一位学者 研究促进奖建议研究性荷尔蒙在 血管内毒素诱导的内毒素休克 细菌、创伤弧菌。要用建议的 实验(编号如下)是不同的荷尔蒙水平 雄性和雌性使雄性极大地增强了对 创伤弧菌内毒素的致命后遗症。这一假设将是 在特定目标测试1，通过实验(1)测试剂量依赖性 雄性和雌性大鼠对创伤弧菌内毒素的反应 实验(2)确定卵巢摘除(OV-x)是否会导致增强 雌性大鼠对内毒素的敏感性，以及实验(3)确定 睾酮进一步增加内毒素对OV-x影响的敏感性 雌性大鼠；在特定目标3，实验(4)如果去势的雄性大鼠 雌二醇-17β对内毒素的反应性降低； 目的4，实验(5)测定一氧化氮的变化 产生改变了大鼠对创伤弧菌内毒素的反应性。

DESCRIPTION (Adapted from the Investigator's Abstract): This is an Academic Research Enhancement Award proposal to study the role of sex hormones in the endotoxic shock induced by lipopolysaccharides (LPS) derived from the bacterium, V. vulnificus. The hypothesis to be tested with the proposed experiments (numbered below) is that different hormonal profiles between males and females predisposes the males to greatly enhanced sensitivity to the virulent sequelae of the V. vulnificus LPS. This postulate will be tested in Specific Aim 1, by experiment (1) testing the dose-dependent responses of male versus female rats to V. vulnificus LPS; in Specific Aim 2, experiment (2) determining whether ovariectomy (ov-x) results in enhanced sensitivity of female rats to LPS, and experiment (3) determining if testosterone further increases sensitivity of LPS-induced effects on ov-x female rats; in Specific Aim 3, experiment (4) if castrated male rats given estradiol-17beta have a decrease in responsiveness to LPS; and in Specific Aim 4, experiment (5) determining whether alteration of nitric oxide production shifts the responsiveness of the rats to V. vulnificus LPS.