PHOSPHORIMAGER SYSTEM

磷光成像系统

• 批准号: 2284259
• 负责人: ALBERT J COUREY
• 金额: $ 8.98万
• 依托单位: UNIVERSITY OF CALIFORNIA LOS ANGELES
• 依托单位国家: 美国
• 财政年份: 1995
• 资助国家: 美国
• 起止时间: 1995-04-01 至 1996-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2284259
• 关键词: Animalia; biomedical equipment purchase; computer graphics /printing; computer program /software; molecular dynamics; phosphorus; radionuclide imaging /scanning

This application requests funds for the purchase of a phosphorimager for UCLA. This instrument is designed to replace conventional X-ray film-based autoradiography for imaging, analyzing and quantitating beta-radiation. The source of radiation is placed in contact with an imaging screen to form a latent image on the screen. The imaging screen is "developed" after an appropriate exposure time by a laser scanner which "releases" the image in the form of photons. Information from the photon detector is stored digitally where it is ready for quantitative analysis or display. The advantages of the phosphorimager over traditional methods are several: 1) increased sensitivity by a factor of 10-200 over traditional methods, 2) a linear response range for data collection that is greater than that for X-ray film by a factor of 10-3 - 10-4, and 3) data storing and processing capabilities that make it possible to objectively quantify signals over the dynamic range of the instrument from a single exposure. These features make previously impractical experiments "doable", they allow the experimenter to extract quantitative information from the stored data and they make it possible to normalize and compare multiple samples. The research descriptions of the major users (Courey - PI - Transcriptional Control of Pattern Formation in the Drosophila Embryo, Berk - Regulation of Transcription by Adenovirus, Clarke - Control of Eukaryotic Membrane Function by Methylation, Gober - Generation of Asymmetry During Caulobacter Development, Gralla - Mechanism of Transcriptional Initiation, Gunsalus - Regulation of Cellular Respiratory Functions, Johnson - Mechanism of site-specific DNA recombination and growth phase regulation by Fis, Merchant - Metalloprotein Biosynthesis in Chlamydomonas, Miller - Invasion of epithelial cells by Yersinia and Salmonella, Morrison - Regulation of Antibody Biosynthesis and Function, Sigman -Chemical Nucleases as Probes of Nucleic Acid Structure-Function Relationships, Simons - Translational Control and RNA Structure, Tobin - Phytochrome Regulation of Specific Gene Expression, Valentine -Functional Studies of Cytosolic Anti-Oxidant Proteins, Weiss - Metabolic Organization in Neurospora) justify the use of the instrument to enhance their research capabilities on each of their NIH-funded research projects. A plan for makihg the instrument available to the large community of researchers in the biomedical sciences at UCLA is in place and resources for its long-term operation and maintenance are guaranteed. The technology is well-developed and has been employed in basic biomedical research for many years. The users include a large group of active investigators, all of whom are engaged in research on health-related, NIH-funded projects representing studies on fundamental aspects of biochemistry, molecular, cell and developmental biology. By enhancing the effectiveness of the research efforts on these individual projects, this application benefits and su orts health-related research.

此应用程序请求资金以购买 加州大学洛杉矶分校的磷光成像仪。此仪器旨在取代 用于成像的传统的基于X射线胶片的放射自显影， 分析和量化贝塔辐射。辐射源 被放置成与成像屏幕接触以形成潜像 在屏幕上。成像屏幕在经过一段时间后才被“显影” 激光扫描仪的适当曝光时间，它将释放 以光子的形式成像。来自光子探测器的信息 以数字形式存储在可以进行定量分析的地方，或者 展示。磷成像仪相对于传统成像仪的优势 方法有几种：1)将灵敏度提高10-200倍 与传统方法相比，2)数据的线性响应范围 收集量比X光胶片大1倍 10-3-10-4和3)数据存储和处理能力 使得能够客观地量化动态上的信号 从一次曝光中获得仪器的范围。这些功能使 以前不切实际的实验是“可行的”，它们允许 实验者从存储的数据中提取定量信息 数据，它们使归一化和比较多个 样本。主要用户(Courey-Pi)的研究说明 -果蝇模式形成的转录调控 胚胎，伯克-腺病毒转录调控，克拉克- 真核细胞膜功能的甲基化调控 不对称性的产生 硫杆菌的发育，Galla-转录机制 启动、冈萨卢斯--细胞呼吸调节 功能，约翰逊--定点DNA重组的机制 以及FIS、Merchant-Metals Protein对生长期的调控 衣藻的生物合成，米勒-上皮的侵袭 耶尔森氏菌和沙门氏菌的细胞--抗体的调节 作为探针的Sigman化学核酸酶的生物合成和功能 核酸结构-功能关系，西蒙斯- 翻译调控与RNA结构、托宾-光敏色素 情人节功能的特异性基因表达调控 胞内抗氧化蛋白Weiss代谢的研究 脉孢子虫的组织)证明使用该仪器是合理的 增强他们在NIH资助的每个项目上的研究能力 研究项目。使仪器可供使用的计划 生物医学科学研究人员的庞大社区位于 加州大学洛杉矶分校为其长期运营和 维护是有保障的。这项技术非常发达， 多年来一直从事基础生物医学研究。这个 用户包括一大群活跃的调查人员，他们都是 致力于NIH资助的与健康相关的项目的研究 代表着对生物化学基本方面的研究， 分子、细胞和发育生物学。通过增强 这些单独项目的研究工作的有效性， 这一应用有利于并支持与健康相关的研究。