BIOLOGICAL FUNCTION OF BASONUCLIN

描述：（改编自申请人的摘要）-长期目标 的建议是了解分子调控机制， 维持细胞的增殖能力和触发信号， 细胞停止增殖。 首席研究员认为， 理解转录调控过程中的过渡， 从增殖到分化是阐明 这些监管程序。 首席研究员最近 发现了一种新的锌指蛋白，basonuclin。 基础核蛋白存在 在表皮增殖区室的角质形成细胞的细胞核中 以及睾丸曲细精管中的生殖细胞。 校长 研究者假设basonuclin是一种转录因子 参与细胞增殖潜能的调节 （能力）。 具体目标1是证明basonuclin是一种DNA， 序列特异性结合蛋白。 方法是选择这些 通过电泳迁移率变动从随机序列库中筛选靶点 使用锌指肽的EMSA测定。 具体目标2将确定 其启动子含有basonuclin靶序列的基因。 校长 研究人员旨在确定由basonuclin控制的基因 从而了解basonuclin是一种 部分 首席研究员将在DNA数据库中搜索 启动子，其含有basonuclin的靶序列，并分析 基因组片段，其根据其结合至所述抗体的能力而被选择。 细菌产生全长的basonuclin。 具体目标3将地图 有丝分裂染色体上的basonuclin结合位点。 联盟 具有有丝分裂染色质的基底核蛋白是非常罕见的。 校长 研究人员的目的是确定有丝分裂染色体， 并克隆这些结合位点，这些结合位点可能是也可能不是 裸DNA上存在相同的结合位点（缺乏组蛋白）， 研究目的2 basonuclin等分子在研究中的优势 生物化学和遗传调控的细胞事件是，它是一个 监管者也被监管。 通过研究，校长 研究者希望不仅获得关于basonuclin的知识， 它所处的监管层级。 校长 研究者认为，拟议的生物学功能研究 basonuclin将提高有关分子调控的知识， 细胞增殖潜能。 他认为，需要这些知识， 为与扩散有关的异常情况设计更好的医疗方法， 皮肤，如癌症、牛皮癣和受损的伤口愈合。 也是 对于设计更好的培养条件以产生大量的 大量的正常人体细胞用于移植和工程目的。

DESCRIPTION: (Adapted from the applicant's abstract) - The long-term goal of the proposal is to understand the molecular regulatory mechanisms that maintain the cellular proliferative capacity and the signals that trigger the cells to cease proliferation. The principal investigator believes that understanding transcriptional regulation during the transition from proliferation to differentiation is an important step toward elucidation of these regulatory processes. The principal investigator has recently discovered a novel zinc finger protein, basonuclin. Basonuclin is present in the nuclei of keratinocytes in the proliferative compartment of epidermis and the germ cells in the seminiferous tubules of testis. The principal investigator hypothesizes that basonuclin is a transcription factor participating in the regulation of cellular proliferative potential (capability). Specific Aim 1 is to demonstrate that basonuclin is a DNA binding protein with sequence specificity. The approach is to select these targets from a pool of random sequences by electrophoresis mobility shift assay (EMSA) using zinc finger peptides. Specific Aim 2 will identify the genes whose promoters contain the basonuclin target sequence. The principal investigator aims to identify the genes that are controlled by basonuclin and thereby to understand the regulatory network in which basonuclin is a part. The principal investigator will search the DNA data base for promoters that contain the target sequence of basonuclin and to analyze the genomic fragments that are selected for their ability to bind to the bacterial produced full length basonuclin. Specific Aim 3 will map basonuclin's binding sites on mitotic chromosomes. The association of basonuclin with mitotic chromatin is highly unusual. The principal investigator aims to identify the mitotic chromosomes that contain basonuclin and to clone these binding sites, which may or may not be the same binding sites present on the bare DNA (lacking histones) that is studied in Aim 2. The virtue of molecules such as basonuclin in the study of biochemical and genetic regulation of cellular events is that it is a regulator that is also regulated. By studying it, the principal investigator expects to gain knowledge not only about basonuclin, but also about the regulatory hierarchy in which it is a part. The principal investigator believes that the proposed study of the biological function of basonuclin will enhance the knowledge about the molecular regulation of cellular proliferative potential. This knowledge is needed, he believes, to devise better medical treatment for proliferation-related abnormalities in the skin, such as cancer, psoriasis, and impaired wound healing. It is also essential for designing better culture conditions to produce large quantities of normal human cells for grafting and engineering purposes.