SPONTANEOUS NMDA CHANNEL ACTIVITY IN HIPPOCAMPAL SLICES

海马切片中的自发 NMDA 通道活动

• 批准号: 2521892
• 负责人: JEFFREY A DZUBAY
• 金额: $ 1.84万
• 依托单位: OREGON HEALTH & SCIENCE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-03-22 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/2521892
• 关键词: NMDA receptors; calcium ion; excitatory aminoacid; glutamates; hippocampus; inhibitor /antagonist; kainate; laboratory rat; membrane channels; membrane potentials; neuropharmacology; neurophysiology; phase contrast microscopy; pyramidal cells; strontium; synapses; tetanus toxin; tissue /cell preparation; voltage /patch clamp

Spontaneous NMDA channel activity has been reported in hippocampal, cortical, and cerebellar slice preparations. The exact source and concentration of the glutamate giving rise to this activity is unknown, as is the location of the receptors. In order to understand the role of NMDA receptors in neuropathies such as epilepsy and excitotoxicty as well as their function in synaptic plasticity and development, it is important to determine the mechanisms of this tonic activation. Specifically: is spontaneous NMDA channel activity associated with spontaneous vesicular release, what role does extrasynaptic glutamate play in this activity, and how long and at what concentration is the glutamate underlying the activity present? To answer these questions, whole cell recordings of pyramidal neurons in transverse hippocampal slices will be taken with the aid of infrared differential interference contrast video microscopy. Using pharmacology and kinetic modeling the specific aims of this proposal will be addressed.

自发的NMDA通道活动已经在海马，