MECHANISM OF A PROTEIN TRANSLOCATION CHANNEL

蛋白质易位通道的机制

• 批准号: 2021499
• 负责人: JONATHAN S ROSENBLUM
• 金额: $ 2.33万
• 依托单位: ROCKEFELLER UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-07-14 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/2021499
• 关键词: cell free system; chloroplasts; lipid bilayer membrane; membrane channels; membrane fusion; membrane potentials; membrane reconstitution /synthesis; protein purification; protein transport

The eukaryotic cell is highly organized, containing organelles that efficiently divide its many tasks. Membranes separate the organelles from one another, sustaining each in a unique chemical environment. In addition, proteinaceous channels exist in the membranes that recognize and transfer certain molecules between the cytosol and a specific organelle. Among the least understood of these channels are those that import proteins bound for certain organelles. The proposed research will use a combination of biochemistry and electrophysiology to identify and characterize the mechanism of proteins composing the outer membrane channel of a model organelle, the chloroplast. The long term objectives of the research are twofold: first, to understand on a molecular level how these channels form a semipermeable barrier between the organelle, and second, to determine how specific recognition of a signal sequence opens a channel.

真核细胞是高度组织化的，含有细胞器， 有效地分配了许多任务。 细胞器被膜隔开 在一个独特的化学环境中维持生命。 此外，蛋白质通道存在于膜中， 识别并在胞质溶胶和细胞质之间转移某些分子。 特殊的细胞器。 其中最不了解的渠道是 那些输入与某些细胞器结合的蛋白质的细胞。 拟议 研究将结合生物化学和电生理学 以确定和表征蛋白质组成的机制， 外膜通道的模式细胞器，叶绿体。 的 研究的长期目标有两个：第一，了解 分子水平上这些通道是如何形成半渗透屏障的 第二，确定细胞器的特异性 信号序列的识别打开通道。