WOUND CONTRACTION--MYOSIN LIGHT CHAIN PHOSPHORYLATION

在儿科手术中有一个长期的观察， 很少出现伤口愈合问题。成人愈合发生在一个 一系列精心安排的事件，其中新的细胞群和 新的基体沉积取代了缺陷，一个不完美的过程举例说明 无法恢复正常真皮的全部拉伸强度。 开放性全层软组织缺损通过该过程修复 在伤口收缩过程中，成纤维细胞与胶原纤维相互作用 产生收缩力。与伤口收缩相反， 疤痕挛缩的并发症，其中收缩的过程 发生在愈合的上皮化伤口中， 机械功能在分子水平上，肌动蛋白- 成纤维细胞微丝中的肌球蛋白丝已经被证明 成为驱动收缩过程的“引擎”。肌动蛋白-肌球蛋白 相互作用依赖于肌球蛋白轻链的磷酸化， 肌球蛋白分子的末端。肌球蛋白轻磷酸化 肌球蛋白轻链激酶（MLCK）的LC 20是 激活细胞微丝收缩。MLCK活性 控制着微丝的收缩通过研究 胎儿和成人成纤维细胞的分子相互作用，这个项目的 具体目的是证明肌球蛋白ATP酶活性的量 与伤口收缩的程度成正比。很长的- 学期目标与本项目的临床意义相一致。的 控制开放性缺损伤口收缩量的能力 改善目前对大面积开放性伤口和烧伤的处理。

There is a long-standing observation in pediatric surgery that a neonate seldom demonstrates wound healing problems. Adult healing occurs by an orchestrated sequence of events in which both new cellular population and new matrix deposition replace a defect, an imperfect process exemplified by the inability to regain the full tensile strength of normal dermis. An open full-thickness soft tissue defect is repaired through the process of wound contraction, in which fibroblasts interact with collagen fibrils producing contractile forces. In contrast to wound contraction is the complication of scar contracture, whereby the process of contraction occurs in healed epithelialized wounds and results in decreased mechanical function. At a molecular level, the interaction of actin- myosin filaments in the microfilaments of fibroblasts has been shown to be the "engine" that drives the contractile process. Actin-myosin interaction depends on the phosphorylation of the myosin light chains at the terminal end of the myosin molecule. Phosphorylation of myosin light chains (LC20) by myosin light chain kinase (MLCK) is required for activation of cell microfilament contraction. The activity of MLCK controls the contraction of microfilaments. Through the study of the molecular interactions of fetal and adult fibroblasts, this project's specific aim is to demonstrate that the amount of myosin ATPase activity is directly proportional to the degree of wound contraction. The long- term goals parallel the clinical implications of this project. The ability to control the amount of contraction in an open defect wound improve current management of large open wounds and burns.