MESOCORTICAL ANATOMY AND PLASTICITY

中皮质解剖学和可塑性

• 批准号: 2037028
• 负责人: GARY W VAN HOESEN
• 金额: $ 22.79万
• 依托单位: UNIVERSITY OF IOWA
• 依托单位国家: 美国
• 财政年份: 1979
• 资助国家: 美国
• 起止时间: 1979-01-01 至 2000-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2037028
• 关键词: Alzheimer's disease; Primates; cerebral cortex; confocal scanning microscopy; entorhinal cortex; fluorescent dye /probe; histochemistry /cytochemistry; human tissue; immunocytochemistry; limbic system; neuroanatomy; neurons; phenotype

This continuation (years 17-20) describes experiments designed to investigate the structural organization of the mesocortices of the limbic lobe in the monkey and human brain. In its initial years, reinnervation of the amygdala was investigated and the term "plasticity" in the title has continued with the grant, although it is not longer descriptive of the research proposed. A central goal has been to investigate cortical association axons that link the association areas with the mesocortices. The reciprocal of this relationship has been investigated as well. In 1989 Alzheimer's disease research was added as a new direction since it targets the exact neurons that mediate this relationship. In the grant's last competitive review dual efforts relating to Alzheimer's disease research and nonhuman primate experimental neuroanatomical research were proposed, and the grant received a Javits Neuroscience Investigator Award. In this proposal, three new aims are outlined. Two deal with combined human and monkey investigations and aim to analyze Brodmann's areas 35 and 34. The former interconnects the entorhinal mesocortices with the temporal isocortices. Area 35 is understood poorly in the human brain and the first to develop neurofibrillary tangles in Alzheimer's disease. Monkey behavioral studies link it closely to associative memory. Area 34 forms the anterior and medial entorhinal cortex and contains laminar alterations in some brains of schizophrenics. It is damaged directly in all types of partial and traumatic herniation of medial temporal lobe through the incisura of the tentorium cerebelli. Cellular phenotypes and boundaries will be established for these areas, focusing both on projection and intrinsic neurons. An additional aim utilizes only the monkey and will assess "three neuron" neural systems related to the mesocortices. Specifically, the terminal fields of entorhinal neurons will be injected with a retrograde tracer and the labeled neurons will be filled intracellularly with Lucifer yellow to reveal their morphology. In the same preparation afferent axons projecting to characterized entorhinal neurons will be anterogradely labeled to establish their putative input patterns to the filled neuron. Confocal laser scanning microscopy will be used to analyze these preparations. For all aims, the data will provide baseline neuroscience information for cortical areas known to be involved in developmental, neurological and psychiatric disease. It is cost effective research that draws on a collection of non-human primate and human material gathered over the past 27 years at Harvard Medical School (1969-1978) and The University of Iowa (1979-present).

这一延续（17-20年）描述了旨在 研究大脑边缘系统中皮层的结构组织 在猴子和人类的大脑中。 在最初的几年里， 的杏仁核进行了调查，并在标题中的术语“可塑性” 继续提供补助金，尽管它不再描述 研究提出。 一个中心目标是研究大脑皮层 连接连接区与中皮层的连接轴突。 也研究了这种关系的倒数。 在 1989年阿尔茨海默病研究被添加为一个新的方向， 它针对的正是调节这种关系的神经元。 在 格兰特的最后一次竞争性审查与阿尔茨海默氏症有关的双重努力 疾病研究和非人灵长类实验神经解剖学 研究被提出，并获得了贾维茨神经科学奖。 调查员奖。 在这一建议中，概述了三个新目标。 其中两项涉及人类和猴子的联合调查，旨在 分析布罗德曼的35和34区 前者将 内嗅中皮层和颞叶等皮层。 区域35 在人类大脑中知之甚少， 阿尔茨海默病的神经系统缠结 猴子行为 研究将其与联想记忆紧密联系起来。 第34章形成 前部和内侧内嗅皮质，并包含层状改变 在精神分裂症患者的大脑中 它是直接损坏的所有类型 内侧颞叶的部分和创伤性疝穿过 小脑幕切迹 细胞表型和 将为这些领域建立边界，重点是 投射和内在神经元。 一个额外的目标只利用 猴子，并将评估“三个神经元”的神经系统相关的 中皮层 具体来说，内嗅神经元的终末区域 将被注射逆行示踪剂，标记的神经元将 细胞内充满了荧光黄， 形态学 在同样的准备中，传入轴突投射到 将顺行标记表征的内嗅神经元， 建立它们对填充的神经元的假定输入模式。 共焦 激光扫描显微镜将用于分析这些制剂。 对于所有目标，数据将提供基线神经科学信息 大脑皮层区域参与了发育，神经， 和精神疾病。 这是一项成本效益高的研究， 收集的非人类灵长类动物和人类的材料， 过去27年在哈佛医学院（1969-1978）和 爱荷华州大学（1979年至今）。