RAB3 FUNCTION IN SYNAPTIC VESICLE TRANSPORT AND FUSION

Rab-3是小GTP结合蛋白家族的成员， 与突触囊泡的运输有关拉布家族 成员和结构上与ras的p2lras产物相关的 GTP和GDP结合形式之间的癌基因循环。每种Rab蛋白 以一种特殊的囊泡运输形式起作用。这种特异性， 其循环机制的调节潜力表明， RAB蛋白在运输调节中的作用。真正的角色， Rab蛋白在转运和膜融合中的作用尚不清楚， 然而.本研究提出Rab3在突触囊泡中的作用， 运输和聚变。实验采用C.优雅作为一个 用于Rab3功能分析的遗传和分子系统。遗传 并且分子方法将首先用于分离新的RAB-3等位基因， 然后通过增强子和转录因子分析Rab3的功能通路， 抑制因子分析这些研究应该产生新的信息， rab-3的功能和突触囊泡的运输。结果 这些实验的结果将有助于剖析一般功能 Rab蛋白和调节其功能的因子。

Rab-3 is a member of a family of small GTP binding proteins which have been implicated in the trafficking of synaptic vesicles. Rab family members and the structurally related to p2lras product of the ras oncogene cycle between GTP- and GDP-bound forms. Each Rab protein appears act in a specific form of vesicle transport. This specificity, together with the regulatory potential of their cycling mechanism suggests a role for RAB proteins in the regulation of trafficking. The actual role which Rab proteins play in the transport and membrane fusion remains a unclear, however. This proposal addresses the role of Rab3 in synaptic vesicle transport and fusion. The experiments presented use C. elegans as a genetic and molecular system for the analysis of Rab3 function. Genetic and molecular methods will first be used to isolate new rab-3 alleles, and then to analyze the Rab3 functional pathway through of enhancer and suppressor analyses. These studies should yield novel information about Rab-3 function and synaptic vesicle transport in general. The results from these experiments will aid in the dissection of the general function of the Rab proteins and the factors modulate their function.