STRUCTURE/FUNCTION OF CAROTENOIDS

这项建议旨在开发一种基于结构的类胡萝卜素模型 在体内参与采光和光保护。他 分离、提纯、重组、交换颜料、结晶 并从基因上改变细菌反应中心和天线 复合体。他使用了X射线衍射、核磁共振和拉曼等工具 光谱学来直接检查结构，还使用EPR和 光谱学。其目的是确定 当类胡萝卜素出现在这些光合作用单位时，产生 全面了解光谱与光谱学的关系 类胡萝卜素的特征和结构，并发现 这些结构之间的关系和 类胡萝卜素在采光和光保护中的作用。这些数据是 预计将提供一个了解更复杂情况的基础 系统；例如，高等植物色素-蛋白质复合体，其性质 比细菌系统更复杂。《公约》的具体目标 建议：(1)研究类胡萝卜素的结构。 光合色素蛋白复合体；(2)探索 类胡萝卜素结构与光谱的关系 性质；(3)确定类胡萝卜素与 捕光和捕光的结构及其机制 光防护功能。

This proposal aims to develop a structurally based model of carotenoid participation in light harvesting and photoprotection in vivo. He isolates, purifies, reconstitutes, exchanges pigments in, crystallizes and genetically modifies bacterial reaction center and antenna complexes. He uses tools such as X-ray diffraction, NMR, and Raman spectroscopy to examine structures directly and also employs EPR and optical spectroscopy. The aim is to determine the structure of carotenoids as they occur in these photosynthetic units, to generate a complete understanding of the relationship between the spectroscopic signatures and structures of the carotenoids and to discover the relations between these structures and the specific roles of the carotenoids in light harvesting and photoprotection. The data are expected to provide a basis from which to understand more complex systems; e.g. higher plant pigment-protein complexes whose properties are more complex than the bacterial systems. The specific aims of the proposal are: (1) To examine the structures of carotenoids in photosynthetic pigment protein complexes; (2) To explore the relationships between the carotenoid structures and their spectroscopic properties; (3) To determine the relationships between the carotenoid structures and their mechanisms of light-harvesting and photoprotection.