STRUCTURE/FUNCTION OF CAROTENOIDS

该提案旨在开发一种基于结构的类胡萝卜素模型 参与体内光捕获和光保护。他 分离、纯化、重构、交换颜料、结晶 并对细菌反应中心和天线进行基因改造 复合物。他使用 X 射线衍射、核磁共振和拉曼等工具 光谱法直接检查结构，还采用 EPR 和 光学光谱学。目的是确定结构 类胡萝卜素出现在这些光合作用单位中时，产生 全面了解光谱之间的关系 类胡萝卜素的特征和结构并发现 这些结构之间的关系以及具体角色 类胡萝卜素在光收集和光保护中的作用。数据是 期望提供一个理解更复杂的基础 系统；例如高等植物色素-蛋白质复合物，其特性 比细菌系统更复杂。该计划的具体目标 建议是： (1) 检查类胡萝卜素的结构 光合色素蛋白复合物； (2) 探索 类胡萝卜素结构与光谱之间的关系 属性； (3) 确定类胡萝卜素之间的关系 光收集的结构及其机制 光保护。

This proposal aims to develop a structurally based model of carotenoid participation in light harvesting and photoprotection in vivo. He isolates, purifies, reconstitutes, exchanges pigments in, crystallizes and genetically modifies bacterial reaction center and antenna complexes. He uses tools such as X-ray diffraction, NMR, and Raman spectroscopy to examine structures directly and also employs EPR and optical spectroscopy. The aim is to determine the structure of carotenoids as they occur in these photosynthetic units, to generate a complete understanding of the relationship between the spectroscopic signatures and structures of the carotenoids and to discover the relations between these structures and the specific roles of the carotenoids in light harvesting and photoprotection. The data are expected to provide a basis from which to understand more complex systems; e.g. higher plant pigment-protein complexes whose properties are more complex than the bacterial systems. The specific aims of the proposal are: (1) To examine the structures of carotenoids in photosynthetic pigment protein complexes; (2) To explore the relationships between the carotenoid structures and their spectroscopic properties; (3) To determine the relationships between the carotenoid structures and their mechanisms of light-harvesting and photoprotection.