STRUCTURE/FUNCTION OF CAROTENOIDS

类胡萝卜素的结构/功能

• 批准号: 2387190
• 负责人: Harry A Frank
• 金额: $ 12.49万
• 依托单位: UNIVERSITY OF CONNECTICUT STORRS
• 依托单位国家: 美国
• 财政年份: 1987
• 资助国家: 美国
• 起止时间: 1987-09-01 至 2001-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2387190
• 关键词: Raman spectrometry; Rhodospirillales; X ray crystallography; bacterial pigment; carotenoids; chemical structure function; cis trans isomerization; electron spin resonance spectroscopy; nuclear magnetic resonance spectroscopy; photochemistry; photoprotection; photosensitizing agents; photosynthetic bacteria; photosynthetic reaction centers; site directed mutagenesis

This proposal aims to develop a structurally based model of carotenoid participation in light harvesting and photoprotection in vivo. He isolates, purifies, reconstitutes, exchanges pigments in, crystallizes and genetically modifies bacterial reaction center and antenna complexes. He uses tools such as X-ray diffraction, NMR, and Raman spectroscopy to examine structures directly and also employs EPR and optical spectroscopy. The aim is to determine the structure of carotenoids as they occur in these photosynthetic units, to generate a complete understanding of the relationship between the spectroscopic signatures and structures of the carotenoids and to discover the relations between these structures and the specific roles of the carotenoids in light harvesting and photoprotection. The data are expected to provide a basis from which to understand more complex systems; e.g. higher plant pigment-protein complexes whose properties are more complex than the bacterial systems. The specific aims of the proposal are: (1) To examine the structures of carotenoids in photosynthetic pigment protein complexes; (2) To explore the relationships between the carotenoid structures and their spectroscopic properties; (3) To determine the relationships between the carotenoid structures and their mechanisms of light-harvesting and photoprotection.

该建议旨在开发一种基于结构的类胡萝卜素模型 参与体内光捕获和光保护。他 分离、纯化、重组、交换色素、结晶 并对细菌的反应中心和触角进行基因改造 配合物他使用X射线衍射、核磁共振和拉曼等工具 光谱直接检查结构，也采用EPR和 光谱学目的是确定 类胡萝卜素，因为它们发生在这些光合单位，以产生 完全理解光谱之间的关系 类胡萝卜素的特征和结构， 这些结构之间的关系和组织的具体作用 类胡萝卜素在光捕获和光保护中的作用。将数据 期望提供一个基础，从了解更复杂的 系统;例如高等植物色素-蛋白质复合物，其性质 比细菌系统更复杂。的具体目标 建议：（1）研究类胡萝卜素的结构， 光合色素蛋白复合物;（2）探讨光合色素蛋白复合物的功能 类胡萝卜素的结构与光谱的关系 （3）确定类胡萝卜素与植物生长的关系 结构及其捕光机制， 光泽全面