AMINO ACID TRANSPORT AFTER SMALL BOWEL RESECTION

小肠切除术后的氨基酸转运

• 批准号: 2770453
• 负责人: HARRY CHARLES SAX
• 金额: $ 11.85万
• 依托单位: UNIVERSITY OF ROCHESTER
• 依托单位国家: 美国
• 财政年份: 1994
• 资助国家: 美国
• 起止时间: 1994-09-30 至 2000-03-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2770453
• 关键词: alanine; aminoacid transport; antiserum; arginine; dietary aminoacid; epidermal growth factor; gastrointestinal absorption /transport; glutamine; histology; hormone regulation /control mechanism; intestinal villi; intestine surgery; jejunectomy /duodenectomy /ileectomy; laboratory rabbit; leucine; neurotensin; nutrition related tag; octreotide; postoperative state; short chain fatty acid; small intestines; somatotropin

DESCRIPTION: (Adapted from investigator's abstract) This research is designed to advance understanding of changes in active amino acid transport by the enterocyte after massive small bowel resection. The role of hormones and specific nutrients as mediators of these-changes will also be examined. The hypotheses to be tested are: 1. Active amino acid transport by the enterocyte is altered during adaptation after massive bowel resection. 2. Specific hormones influence amino acid transport during this adaptation. 3. Specific nutrients, including amino acids and short chain fatty acids (SCFA) may be beneficial after resection by improving amino acid uptake. New Zealand rabbits undergo 70% jejunoileal resection or sham operation. One week, one month, and six months later, small bowel mucosa will be processed to brush border membrane vesicles. Uptake of glutamine (GLN), arginine, alanine, and leucine will be quantified using a rapid mixing/filtration technique. Changes in plasma levels of neurotensin (NT) epidermal growth factor (EGF), and growth hormone (GH) will be determined by radioimmunoassay. Infusions of NT, EGF, GH and the somatostatin analogue octreotide will assess hormonal influence on amino acid transport. Octreotide's effect will be further verified by immuno- neutralization with specific antisera to NT, EGF, and GH. Dietary manipulation includes the provision of isonitrogenous, isocaloric diets with protein as whole protein, oligopeptides or free amino acids. SCFA will be supplemented either as free sodium butyrate or pectin. Influence of parenteral GLN on amino acid transport from both the lumen and the splanchnic circulation will also be quantified. The eventual goal is to develop nutrient/hormone combinations that will improve adaptation. It is hoped that the knowledge gained will contribute to new therapeutic strategies for patients with short gut syndrome.

描述：（改编自研究者摘要）本研究是

项目成果

Octreotide diminishes luminal nutrient transport activity, which is reversed by epidermal growth factor.

奥曲肽可减少管腔营养物质转运活性，表皮生长因子可逆转这种活性。

• DOI: 10.1016/s0002-9610(96)00163-8
• 发表时间: 1996
• 期刊: American journal of surgery
• 影响因子: 3
• 作者: Seydel,AS;Miller,JH;Sarac,TP;Ryan,CK;Chey,WY;Sax,HC
• 通讯作者: Sax,HC

Glucocorticoids upregulate intestinal nutrient transport in a time-dependent and substrate-specific fashion.

• DOI: 10.1016/s1091-255x(98)80036-x
• 发表时间: 1998-09-01
• 期刊: Journal of gastrointestinal surgery : official journal of the Society for Surgery of the Alimentary Tract
• 作者: Iannoli, P;Miller, J H;Sax, H C
• 通讯作者: Sax, H C

Characterization of L-leucine transport system in brush border membranes from human and rabbit small intestine.

人和兔小肠刷状缘膜中 L-亮氨酸转运系统的表征。

• DOI: 10.1016/s0026-0495(99)90155-5
• 发表时间: 1999
• 期刊: Metabolism: clinical and experimental
• 作者: Iannoli,P;Miller,JH;Wang,HT;Bode,B;Souba,WW;Avissar,NE;Sax,HC
• 通讯作者: Sax,HC

Low-dose enteral feeding is beneficial during total parenteral nutrition.

全肠外营养期间低剂量肠内喂养是有益的。

• DOI: 10.1016/s0002-9610(96)00039-6
• 发表时间: 1996
• 期刊: American journal of surgery
• 影响因子: 3
• 作者: Sax,HC;Illig,KA;Ryan,CK;Hardy,DJ
• 通讯作者: Hardy,DJ

Early nutritional support in critical illness is important.

危重疾病的早期营养支持很重要。

• DOI: 10.1016/s0749-0704(05)70269-4
• 发表时间: 1996
• 期刊: Critical care clinics
• 影响因子: 4.3
• 作者: Sax,HC