14-3-3 PROTEIN FUNCTION IN CELL GROWTH AND MOTILITY

描述：本提案的核心是理解 14-3-3信号分子。主要目标是建立14-3-3 特异性地结合丝氨酸磷酸化的蛋白并帮助组装 信号复合体，调节细胞的增殖、分化和 细胞运动性。这一假设将通过三个具体的 实验目标。第一个是评估14-3-3的能力 与体内特定的磷酸丝氨酸基序结合(通过使用突变体 Raf-1激酶的形式)。第二个目标侧重于考察 14-3-3在Raf-1介导的信号转导中的作用 特异性14-3-3抑制剂对非洲爪哇胚胎发育的影响 第三个目标将集中在研究14-3-3在 培养过程中作用聚合和细胞运动的调节 成纤维细胞。预计这些实验的结果将 确定14-3-3信号分子在细胞生长中的重要性 规范和发展。

DESCRIPTION: This proposal centers on understanding the function of the 14-3-3 signaling molecule. The major goal is to establish that 14-3-3 specifically binds to serine-phosphorylated proteins and helps to assemble signaling complexes that regulate cell proliferation, differentiation, and cell motility. This hypothesis will be tested through three specific experimental aims. The first will be to assess the ability of 14-3-3 to bind to specific phosphoserine motifs in vivo (through the use of mutant forms of the Raf-1 kinase). The second aim focuses on examining the role of 14-3-3 in Raf-1-mediated signal transduction through an analysis of the effects of specific 14-3-3 inhibitors on Xenopus laevis embryo development. The third aim will then center on examining the role of 14-3-3 in the regulation of acting polymerization and cell motility in cultured fibroblasts. The expectation is that the results of these experiments will establish the importance of the 14-3-3 signaling molecules in cell growth regulation and development.