PATTERNING GENES IN RETINAL DEVELOPMENT

视网膜发育中的基因模式

• 批准号: 2603438
• 负责人: Deborah L Stenkamp
• 金额: $ 11.3万
• 依托单位: UNIVERSITY OF IDAHO
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-06-01 至 2003-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2603438
• 关键词: Osteichthyes; alternatives to animals in research; biological signal transduction; cell cycle; cell differentiation; cytogenetics; developmental genetics; gene expression; genetic regulation; goldfish; histogenesis; immunocytochemistry; in situ hybridization; microinjections; retina; retinal pigment epithelium; rod cell; visual photoreceptor; zebrafish

DESCRIPTION (Adapted from applicant's abstract): The long-range objective of the proposed research is to determine the cellular and molecular developmental events that lead to the differentiation of specific cell types in the vertebrate retina. The immediate experimental approach toward this goal is to focus on the role of hh genes in retinal development in teleost fish. Five specific aims are proposed: (1) to characterize the spatiotemporal expression of hh genes in the retinal pigmented epithelium (RPE) of zebrafish; (2) to experimentally reduce expression of hh genes in the RPE of embryonic zebrafish and examine the effects on retinal cell differentiation and patterning; (3) to create sites of precocious and/or ectopic hh expression in the zebrafish eye and examine the effects on retinal cell differentiation and patterning; (4) to examine specific effects of hh protein on rod photoreceptor production in the retina of adult goldfish; and (5) to identify retinal cell types that receive the hh signal by localizing expression of the hh receptor. The proposed research program emphasizes potential involvement of hh gene expression in the process of rod photoreceptor development. A loss of rod photoreceptors is a primary event in a number of hereditary retinal degenerations; the study of genes that may be involved in rod production may ultimately lead to the development of strategies for rod replacement therapy in humans.

描述（改编自申请人的摘要）：长期目标 拟议研究的目的是确定细胞和分子 导致特定细胞类型分化的发育事件 在脊椎动物的视网膜中。 对此的直接实验方法 目标是关注 hh 基因在硬骨鱼视网膜发育中的作用 鱼。 提出了五个具体目标：（1）描述 视网膜色素上皮中hh基因的时空表达 斑马鱼的（RPE）； (2) 实验性地减少 hh 基因的表达 胚胎斑马鱼的 RPE 并检查对视网膜细胞的影响 差异化和模式化； (3) 创建早熟网站和/或 斑马鱼眼中的异位 hh 表达并检查其影响 视网膜细胞分化和模式化； (4)检验具体效果 hh 蛋白对成人视网膜视杆光感受器产生的影响 金鱼; (5) 识别接收 hh 信号的视网膜细胞类型 通过 hh 受体的局部表达。 拟议的研究计划 强调 hh 基因表达在杆状细胞过程中的潜在参与 光感受器发育。 视杆细胞感光细胞的丧失是主要事件 多种遗传性视网膜变性；对基因的研究可能 参与棒材生产可能最终会导致开发 人类杆替代疗法的策略。