CELL DEATH IN EMBRYONIC HEAD MUSCLES

鸡胚胎头面部肌肉发育的研究进展 意外发现中途初级肌管广泛变性 通过胚胎期。肌肉细胞死亡(细胞凋亡)是 仅限于在这些胚胎中发育头部和颈部肌肉， 提示细胞凋亡在建立适当的 头面部肌肉的功能。S的缘起和意义 发育中的肌肉细胞的程序性细胞死亡尚不清楚。这个 这里提出的研究将定义程序性细胞死亡的程度 在选定的头部和颈部肌肉中，并将调查可能的联系 纤维类型和肌细胞存活率之间的关系。亲切的细胞死亡 运动神经元已经得到了很好的研究，但神经元变性的影响 在体内对初级肌管的作用尚不清楚。这将由以下人员进行分析 减少运动神经元细胞死亡的程度，并检查 肌肉。最后，细胞死亡可能在特定的生肌细胞中被编程。 血统。血统依赖的细胞凋亡将通过移植进行评估 非凋亡性谱系取代了凋亡性肌肉祖细胞。 这些实验将阐明细胞凋亡的原因(S)和作用 发育的头面部肌肉。

Recent studies of craniofacial muscle development in chick embryos unexpectedly revealed extensive degeneration of primary myotubes midway through the embryonic period. Muscle cell death (apoptosis) is restricted to developing head and neck muscles in these embryos, suggesting that apoptosis has a role in establishing appropriate functions of craniofacial muscles. The cause(s) and significance of programmed cell death of developing muscle cells are not understood. The research proposed here will define the extent of programmed cell death in selected head and neck muscles, and will investigate possible links between fiber type and muscle cell survival. Pro- graced cell death of motor neurons is well-studied, but the impact of neuronal degeneration on primary myotubes in vivo is not known. This will be analyzed by reducing the extent of motor neuron cell death, and examining the muscles. Finally, cell death may be programmed in specific myogenic lineages. Lineage-dependent apoptosis will be assessed by transplanting non-apoptotic lineages in the place of apoptotic muscle progenitors. These experiments will elucidate the cause(s) and role of apoptosis in developing craniofacial muscles.