COVID-19: APTAMER-BASED DIAGNOSTICS KIT FOR THE EARLY AND RAPID DETECTION OF COVIDS

COVID-19：基于适配体的诊断试剂盒，用于早期快速检测新冠病毒

• 批准号: 72889
• 金额: $ 14.6万
• 依托单位: TISSUE CLICK LTD
• 依托单位国家: 英国
• 项目类别: Collaborative R&D
• 财政年份: 2020
• 资助国家: 英国
• 起止时间: 2020 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=72889
• 关键词: COVID; 19; APTAMER; BASED; DIAGNOSTICS

APTA-COVID aims to develop **a user-friendly kit** for the early and fast diagnosis of COVID (and its future mutants) infection. Computer modelling will enable the identification of a library of relatively small molecules (called aptamers) able to recognise different domains of the COVID-19 spikes. These molecules will be grafted on to magnetic nanoparticles to become **a powerful tool to capture, concentrate and detect small amounts of viruses in oral swabs/saliva samples**. This will be done by incubating the magnetic nanoparticles with the sample for only 15 min, attracting them with a magnet and after washing out the excess, incubate them for 15 min with free aptamers tagged with fluorescent molecules: each recognising a different virus spike domain. After a final wash, particles that fluoresce under illumination with an inexpensive UV torch will signal the presence of the virus. This kit will be much more cost-effective than the one currently available (£50 for 1000 samples). More individuals will be tested in a shorter time period thus enabling earlier detection of infection and geographical mapping of outbreaks enabling fast actions by clinicians and policymakers.

APTA-COVID旨在开发 ** 一种用户友好的试剂盒 **，用于COVID（及其未来突变体）感染的早期和快速诊断。计算机建模将能够识别相对较小的分子（称为适体）库，这些分子能够识别COVID-19尖峰的不同结构域。这些分子将被嫁接到磁性纳米颗粒上，成为一种强大的工具，用于捕获、浓缩和检测口腔拭子/唾液样本中的少量病毒。这将通过将磁性纳米颗粒与样品孵育仅15分钟来完成，用磁体吸引它们，并在洗掉多余的之后，将它们与标记有荧光分子的游离适体孵育15分钟：每个识别不同的病毒刺突结构域。最后一次清洗后，在廉价的紫外线手电筒照射下发出荧光的颗粒将发出病毒存在的信号。这个工具包将比目前可用的工具包更具成本效益（1000个样本50英镑）。更多的人将在更短的时间内接受检测，从而能够更早地发现感染，并绘制疫情的地理分布图，使临床医生和政策制定者能够迅速采取行动。