NEW METHOD FOR MEASUREMENT OF CDT LEVEL IN SERUM

测量血清中 CDT 水平的新方法

• 批准号: 2422304
• 负责人: BORIS Y ZASLAVSKY
• 金额: $ 9.98万
• 依托单位: ANALIZA, INC.
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-09-25 至 1998-09-24
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2422304
• 关键词: affinity chromatography; alcoholism /alcohol abuse; analytical method; biomarker; densitometry; diagnosis design /evaluation; electrofocusing; enzyme linked immunosorbent assay; human tissue; immunologic assay /test; method development; serology /serodiagnosis; transferrin; western blottings

DESCRIPTION: (Adapted from the applicant's abstract) The innovation described in this proposal is aimed at providing a new method for detection of carbohydrate-deficient transferrin (CDT) in human blood as a biochemical marker of alcohol abuse. CTD has been extensively shown to be a sensitive and specific marker related to long term excessive alcohol consumption. Existing analytical techniques for the measurements of CTD in the blood or its ration to intact transferrin involve separation of CTD from other blood proteins, followed by concentration measurement typically using immunoassay methods. These techniques are in general time consuming, expensive, and must be performed by trained professionals. ANALIZA is proposing a new technique for the measurement of CDT/transferrin ratio directly using blood samples without pre- separation. The technique is based upon a novel application of aqueous two-phase partitioning. The technique is unique in the type of information obtained, is inexpensive and time-and labor-efficient, and may provide additional information not possible with existing technologies. Phase I work will involve the design and analysis of the test and its preliminary feasibility and validation study. Phases II and III work will concentrate on a wide-range validation protocol, together with optimization studies and a construction of a prototype automated instrument.

描述：（改编自申请人的摘要） 本提案中描述的目的是提供一种新的方法， 检测人血液中的碳水化合物缺乏型转铁蛋白（CDT）， 酒精滥用的生化标志CTD已被广泛证明 是长期过量饮酒的敏感和特异性标志物 消费用于测量CTD的现有分析技术 在血液或其定量完整的转铁蛋白涉及分离， 其他血液蛋白的CTD，随后进行浓度测量 通常使用免疫测定方法。这些技术在一般情况下 消耗、昂贵，并且必须由受过训练的专业人员执行。 ANALIZA提出了一种新的技术，用于测量 CDT/转铁蛋白比率直接使用血液样品，无需预处理。 分居该技术是基于一种新的应用水 两相分离该技术是独特的类型， 所获得的信息成本低廉，省时省力， 可能提供现有技术无法提供的额外信息 技术.第一阶段的工作将包括设计和分析 试验及其初步可行性和验证研究。第二阶段和 III的工作将集中在一个广泛的验证协议， 通过优化研究和自动化原型的构建， 仪器