PHOTODYNAMIC TREATMENT OF STAPHYLOCOCCUS INFECTIONS

最常见的是金黄色葡萄球菌伤口感染 发生在人类身上的感染导致延迟和严重的伤口 治愈、败血症和死亡。它们是最常见的致病原因 急性伤口感染和慢性伤口感染中的细菌。 不幸的是，普通人群中的金黄色葡萄球菌 对所有常用的口服抗生素产生了显著的耐药性 90%以上的菌株对甲氧西林耐药。 此外，用波伏丁擦洗伤口的标准技术- 碘溶液对葡萄球菌菌落数无影响 伤痕累累的金色皮肤。 光动力疗法(PDT)是一种涉及使用 光敏剂和激光产生氧自由基 在摧毁癌细胞、细菌、病毒和真菌方面。 医疗实践中常用的几种染料都很好。 光动力试剂，即亚甲基蓝和甲苯蓝。这些 与适当波长的光相结合的试剂 导致对抗生素耐药的金黄色葡萄球菌的破坏。 建议的研究将尝试建立光动力疗法的可行性。 用局部染料作为一种杀菌手段来治疗急性疾病 动物模型中的感染伤口，展示了其有效性， 这种治疗方法简单易行，发病率极低。 建议的商业应用 拟议研究的长期目标是开发光动力疗法。 使用外用染料作为主要防腐处理的治疗装置 对于急性伤口以及对慢性感染的治疗 伤口。因为这种疗法的作用机制不同于 局部和全身抗生素，它也会减少显著的 产生抗生素耐药性的问题。

Staphylococcus aureus wound infections are the most common infections that occur in humans resulting in delayed and poor wound healing, sepsis and death. They are the most common causative bacteria in acute wound infections as well as chronic wound infections. Unfortunately, Staphylococcus aureus in the general population has developed significant resistance to all commonly used oral antibiotics with over 90 percent of strains being methicilllin resistant. Furthermore, standard scrubbing techniques of wounds with povodine- iodine solutions do not affect the bacterial counts of staphylococcus aureus in open wounds. Photodynamic therapy (PDT) is a modality that involves the use of a photosensitizing agent and laser light to create oxygen radicals resulting in the destruction of cancer cells, bacteria, viruses as well as fungi. Several dyes commonly used in medical practice are excellent photodynamic agents ie, methylene blue and toluidene blue. These agents in combination with the appropriate wavelength of light have resulted in the destruction of antibiotic resistant staphylococcus aureus. The proposed research will attempt to establish the feasibility of PDT treatment with topical dyes as a means to antiseptically treat acutely infected wounds in an animal model, demonstrating the effectiveness, ease and minimal morbidity of this treatment. PROPOSED COMMERCIAL APPLICATION The longterm objective of the proposed research is to develop PDT treatment devices with topical dyes as a primary antisepsis treatment for acute wounds as well as a treatment for chronically infected wounds. As this treatment has a different mechanism of action than topical and systemic antibiotics, it will also reduce the significant problem of developing antibiotic resistance.