ARROW, A NEW WINGLESS SIGNALING COMPONENT

ARROW，一种新型无翼信号组件

• 批准号: 2673395
• 负责人: Kim A Caldwell
• 金额: $ 1.87万
• 依托单位: COLUMBIA UNIV NEW YORK MORNINGSIDE
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-09-01 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/2673395
• 关键词: Drosophilidae; developmental genetics; invertebrate embryology; molecular cloning

The long-term objectives of this work are to elucidate the molecular basis of pattern formation during development in Drosophila melanogaster. The characterization of a genetic component of the Wingless signaling pathway in the Drosophila embryonic epidermis will serve to define elements required for regulation of developmental decisions in invertebrates and, by analogy, vertebrates. The molecular cloning of arrow, a newly identified member of the Wingless pathway, will be performed and will facilitate subsequent studies designed to further identify the role arrow plays in the Wingless pathway. The generation of antibodies specific to the Arrow protein will aid in determining its cellular localization. Genetic epistasis and embryonic microinjection will provide information regarding the location, and possibly the mechanism of action, of arrow in the Wingless pathway. The implications of this work lie in the possibility that this genetic cascade will provide insights into mechanisms of vertebrate development as the Wingless pathway utilizes a novel form of signal transduction and is highly conserved evolutionarily.

这项工作的长期目标是阐明 黑腹果蝇发育模式形成的基础。 无翅信号的遗传成分的表征 果蝇胚胎表皮中的信号通路将用来定义 发展决策监管所需的要素 无脊椎动物，以此类推，脊椎动物的分子克隆 箭头，一个新发现的成员无翼途径，将是 进行，并将促进后续研究旨在进一步 确定箭头在无翼路径中的作用。的产生 针对Arrow蛋白的抗体将有助于确定其 细胞定位遗传上位性与胚胎显微注射 将提供有关位置的信息，并可能 无翼通道中的箭的作用机制。的影响 这项工作的关键在于这种遗传级联将 提供了深入了解脊椎动物发展的机制， 无翅途径利用了一种新的信号转导形式， 在进化上高度保守