SOMATIC STEM CELL REGULATION IN THE DROSOPHILA GERMARIUM

这里提出的研究的长期目标是了解如何 生长、分化和图案化过程在体内进行，使用 果蝇生殖室中的体细胞干细胞增殖(位于 每个卵巢的顶端)作为一个模型系统。之前的实验已经 建议刺猬(HH)在加工过程中发挥独特的作用。因此，要 确定胚胎室中的体细胞增殖是否 绝对依赖于HH并在需要其表达的情况下，FLP- OUT技术将用于生成空的HH克隆。识别单元格的步骤 作为对HH的回应，标记的细胞克隆将在野生型中进行分析 对HH--过度表达的细菌。此外，还有两个备选方案 建议采用(分子或基因筛查)方法进行鉴定 与体细胞增殖调控有关的其他基因 生殖器。进一步分析分离基因的潜在作用 在对照中，体细胞增殖将在 这项研究金的任期将在以后的阶段继续。这个的用法 强大的基因系统识别控制细胞的因素 体内增殖分化及其作用机制的研究 对于理解这种监管的扰乱将是有价值的 加工过程会导致癌症等病理情况，并可能 提供对治疗策略的见解。

The long term objective of the research proposed here is to understand how growth, differentiation and patterning processes take place in vivo, using somatic stem cell proliferation in the Drosophila germarium (located at the tip of each ovariole) as a model system. Previous experiments have suggested a unique role for hedgehog (hh) in process. Therefore, to determine whether somatic cell proliferation in the germarium is absolutely dependent on hh and where its expression is necessary, the FLP- out technique will be used to generate null hh clones. To identify cells responsive to hh, marked clones of cells will be analyzed in wild-type versus hh--overexpressing germarium. In addition, two alternative approaches (a molecular or a genetic screen) are proposed to identify other genes involved in the control of somatic cell proliferation in the germarium. Further analysis of the potential role of the isolated genes in the control somatic cell proliferation will be initiated during the term of this fellowship and continued at a later stage. The use of this powerful genetic system to identify factor which control cell proliferation-differentiation in vivo and study their mechanisms of action will be valuable to understand how the disruption of such regulatory processes results in pathological conditions such as cancers and may provide insights into therapeutic strategies.