DEVELOPMENT OF A MAMMALIAN CELL AGING MACHINE

这个试点项目的目标是构建和评估一种新的文化 哺乳动物细胞的这项技术被称为“衰老机器”。新的 老年机与最初的蜂窝婴儿机相似之处在于它 包括将细胞群固定在表面上，从而 作为分裂的结果，新生细胞从表面脱落 附着的细胞。与老化机器的区别在于， 细胞通过差压等方式附着在表面上 这种连接是永久性的，两个姐妹细胞中的一个形成了 总会被释放的。因此，新生的细胞 无限期释放，实现对细胞周期的自动分析，以及 许多其他细胞生长特性，作为年龄的函数 连接的细胞。试点研究支持将用于组装 并检测固定化细菌的生长特性。 和释放的细胞，包括生长速度和质量同步 生长，在仪器中生长的持续时间，以及最大细胞 收益率。这些测试最初将在小鼠L1210细胞上进行，但 对其他细胞类型的应用也将进行评估。的效用 用于研究细胞周期参数和细胞分裂的仪器 细胞分裂时的成分与复制衰老的关系 被建立起来。老化机的主要优点之一是 它允许细胞周期研究基本上不受干扰，或者非常 最小的干扰，种群。如果仪器操作起来也很容易 不出所料，它可以用于常规实验室 在连续培养系统中维持细胞群体 还会在整个生命周期内自动生成新生细胞 固定的种群。

The goal of this pilot project is to construct and evaluate a new culture technique for mammalian cells called the "aging-machine". The new aging-machine is similar to the original cellular baby-machine in that it involves the immobilization of a cell population on a surface such that newborn cells are shed from the surface as a consequence of division of the attached cells. The difference with the aging-machine is that the cells are attached to the surface by a means, differential pressure, such that the attachment is permanent and one of the two sister cells formed at division is always released. As a consequence, newborn cells are released indefinitely, enabling automatic analysis of the cell cycle, and numerous other cell growth properties, as a function of the age of the attached cells. The pilot research support will be used to assemble the instruments and to examine the growth characteristics of the immobilized and released cells, including growth rates and quality of synchronous growth, the duration of growth in the instruments, and the maximum cell yields. The tests will be performed initially with mouse L1210 cells, but applications to other cell types will also be evaluated. The Utility of the instruments for investigating cell cycle parameters and partitioning of components between cells at division in relation to replicative aging will be established. One of the major advantages of the aging-machine is that it permits cell cycle studies on essentially undisturbed, or very minimally disturbed, populations. If the instrument operates as easily and as well as anticipated, then it could be used for routine laboratory maintenance of cell populations in a continuous culture system which also automatically generates newborn cells throughout the lifespan of the immobilized population.