DIAGNOSIS AND CONTROL OF SIMIAN VARICELLA EPIZOOTICS

描述(改编自申请人的摘要)：SVV引起高度 非人类灵长类动物中的传染性、危及生命的疾病。这很自然 东半球猴病的特征是发烧、水疱性皮疹、 而且常常是传播性疾病，包括肝炎和肺炎。一次 最初，这种疾病可能会迅速传播到整个灵长类群体 易受影响的猴子。几家主要的猿猴水痘流行性出血热 在过去的三十年里，在灵长类设施中零星发生。 在圈养的非人类人群中，这些流行性疾病涉及高 发病率高，有时死亡率高，导致研究资源损失 和有价值的研究数据。适当的抗病毒治疗以控制 如果疾病被迅速诊断，家禽流行性感冒是可能的。不幸的是， 猴水痘的快速诊断方法不是 目前可用。这个应用程序的总体目标是开发 快速和特异的诊断分析，这将有助于控制 灵长类设施中的SVV流行性感冒。免疫组织化学检测将会 旨在检测可接触的组织中的特定SVV抗原 感染了SVV的猴子。利用聚合酶链式反应(PCR)进行检测 将被开发用于检测受感染猴子组织中的SVV核酸。 此外，还将开发一种酶联免疫分析(ELISA)，以 检测感染猴血清中的SVV抗原抗体 目的：评估SVV暴露与猴水痘易感性。 本试验将用于进行猿猴的流行病学调查 中华人民共和国的水痘。

DESCRIPTION (Adapted from the applicant's abstract): SVV causes a highly contagious, life-threatening disease in nonhuman primates. This natural disease of Old World monkeys is characterized by fever, vesicular skin rash, and often disseminated disease, including hepatitis and pneumonia. Once initiated, the disease may rapidly spread throughout a primate colony to susceptible monkeys. Several major simian varicella epizootics have occurred sporadically in primate facilities over the past three decades. These epizootics in captive nonhuman populations have involved high morbidity and sometimes high mortality, resulting in lost research resources and valuable research data. Appropriate antiviral therapy to control epizootics is possible if the disease is quickly diagnosed. Unfortunately, diagnostic methods for the rapid diagnosis of simian varicella are not currently available. The overall goal of this application is to develop rapid and specific diagnostic assays which will be useful for the control of SVV epizootics in primate facilities. Immunohistochemical assays will be designed to detect specific SVV antigens in accessible tissues in SVV-infected monkeys. Assays utilizing the polymerase chain reaction (PCR) will be developed to detect SVV nucleic acid in tissues of infected monkeys. In addition, an enzyme-linked immunoassay (ELISA) will be developed to detect antibodies to SVV antigens in sera derived from infected monkeys in order to evaluate exposure to SVV and susceptibility to simian varicella. This assay will be used to conduct an epidemiological survey of simian varicella at the TRPRC.