MINORITY PREDOCTORAL FELLOWSHIP PROGRAM

少数族裔博士前奖学金计划

• 批准号: 2545198
• 负责人: Melinda Mary Pettigrew
• 金额: $ 3.31万
• 依托单位: YALE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-10-01 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/2545198
• 关键词: Aedes; Bunyaviridae; communicable disease control; genetically modified animals; insect control; minority health professional

The major goal of my thesis project is to test the feasibility of creating a transgenic strain of the mosquito Aedes triseriatus which shows a reduced ability to transmit LaCrosse virus (LAC). LAC virus is a member of the California serogroup of bunyaviruses, and is a major cause of mosquito borne encephalitis in children. The project will involve the selection of neutralizing monoclonal antibodies to LAC, and the expression of selected single chain antibody fragments in the mosquito using a double subgenomic Sindbis virus expression system. The Sindbis recombinants will be evaluated to determine if the expressed antibody fragment is capable of inhibiting productive viral infection or blocking the transmission of LAC. A secondary aim of the project is to design a transformation system for the bacterial endosymbiont Wolbachia. Wolbachia is associated with the phenomenon of cytoplasmic incompatibility. This bacteria could be used to introduce and maintain the desired refractory genes in insect populations.

我的论文项目的主要目标是测试的可行性 创造了三列伊蚊的转基因品系， 显示传播曲棍球病毒 (LAC) 的能力降低。 LAC病毒是 加州布尼亚病毒血清群的成员，是一种主要的 引起儿童蚊媒脑炎的原因。该项目将 涉及针对 LAC 的中和单克隆抗体的选择，以及 所选单链抗体片段的表达 使用双亚基因组辛德比斯病毒表达系统的蚊子。这 将评估 Sindbis 重组体以确定是否表达 抗体片段能够抑制产生性病毒感染或 阻断LAC的传播。该项目的第二个目标是 设计细菌内共生体沃尔巴克氏体的转化系统。 沃尔巴克氏体与细胞质现象有关 不兼容。这种细菌可用于引入和维持 昆虫种群中所需的耐药基因。