GENETIC BIOMARKERS WITH CALIFORNIA SEA LIONS

加州海狮的基因生物标志物

• 批准号: 2545993
• 负责人: DARLENE A HASTINGS
• 金额: $ 1.48万
• 依托单位: UNIVERSITY OF TEXAS MED BR GALVESTON
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-09-30 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/2545993
• 关键词: Pinnipedia; benzopyrenediol epoxide; carbopolycyclic compound; chlorohydrocarbon; environmental toxicology; gene environment interaction; gene frequency; gene mutation; genetic markers; lymphocyte; marine biology; water pollution

Pollution of marine environments near human population centers can expose marine mammals to carcinogenic and mutagenic chemicals. Humans residing near these areas may share some of the same food sources, exposing them to similar risks. We propose to adapt an established method for monitoring human populations for mutagen exposure, the autoradiographic hprt mutant lymphocyte assay, for use with California sea lions (Zalophus californianus). Assay conditions and baseline mutant frequencies will be established with blood samples obtained from a captive population at Sea World, San Antonio, Tx. As a "positive" control, lymphocytes from this population will be exposed to a reactive polyaromatic hydrocarbon, such as a benz(a)pyrene diol - epoxide, in vitro to determine their sensitivity to a characteristic environmental mutagen. Human lymphocytes will also be treated in vitro for comparison. Blood samples from 15-20 stranded free- living sea lions will be obtained from the Marine Mammal Center in Sausalito, CA. Lymphocytes will be assayed for frequencies of hprt mutants. Data on tissue levels of polycyclic aromatic hydrocarbons and organochlorine levels, as determined by the Marine Mammal Center, will be used to analyze the relationship between mutant frequency and exposure.

人类聚居地附近的海洋环境污染会使 海洋哺乳动物致癌和致突变化学品。人类居住 这些地区附近的动物可能分享一些相同的食物来源，使它们暴露于 类似的风险。我们建议采用一种既定的监测方法， 暴露于诱变剂的人群，放射自显影hprt突变体 淋巴细胞测定，用于加州海狮（Zalophus californianus）。试验条件和基线突变频率将 用从海上捕获的人口中获得的血液样本建立 世界，圣安东尼奥，TX.作为“阳性”对照， 人口将暴露于反应性多环芳烃，如 苯并（a）芘二醇-环氧化物，在体外，以确定其敏感性， 一种典型的环境诱变剂人体淋巴细胞也将 在体外进行处理以进行比较。从15-20个被困的自由人身上采集的血样- 活海狮将从海洋哺乳动物中心获得， 加利福尼亚州索萨利托。将测定淋巴细胞的hprt突变体频率。 关于多环芳烃组织水平的数据， 海洋哺乳动物中心测定的有机氯含量将被 用于分析突变频率与暴露之间的关系。