PUROMYCIN SENSITIVE (ENKEPHALIN) AMINOPEPTIDASE ISOFORMS
PUROMYCIN SENSITIVE (ENKEPHALIN) AMINOPEPTIDASE ISOFORMS
批准号:
2517895
负责人:
MICHAEL W THOMPSON
金额:
$1.49万
依托单位:
依托单位国家:
美国
项目类别:
财政年份:
1997
资助国家:
美国
项目状态:
未结题
起止时间:
1997-09-01 至
中文摘要
本研究计划的目的是研究表达和
英文摘要
The objective of this research proposal is to study the expression and
regulation of the puromycin-sensitive aminopeptidase (enkephalin-
degrading aminopeptidase), which has been shown to degrade neuropeptides
including the enkephalins and dynorphins. Recently, three isoforms of
this enzyme were isolated from a cDNA library. We will utilize these
cDNAs to isolate a genomic clone(s) of the enzyme to determine the origin
of these isoforms. It is expected that the three isoforms arise from a
single gene due to the near identity of the majority of their coding
sequences. Exons of the genomic clone will be sequenced to determine
their splicing pattern. We will determine how the enzyme is regulated in
cells, including where each isoform is located in the cell, whether or
not each isoform is catalytically active, and how these isoforms are
expressed spatially and temporally. Finally, transgenic mice will be
employed to study the putative role of the enzyme isoforms on development
and on regulation of the cell cycle.
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PUROMYCIN SENSITIVE (ENKEPHALIN) AMINOPEPTIDASE ISOFORMS
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批准号:6135738
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项目类别:
-
资助金额:$1.81万
-
财政年份:1998
-
负责人:MICHAEL W THOMPSON
-
依托单位:
PUROMYCIN SENSITIVE (ENKEPHALIN) AMINOPEPTIDASE ISOFORMS
-
批准号:2770064
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项目类别:
-
资助金额:$1.51万
-
财政年份:1998
-
负责人:MICHAEL W THOMPSON
-
依托单位:
PUROMYCIN SENSITIVE (ENKEPHALIN) AMINOPEPTIDASE ISOFORMS
-
批准号:2118244
-
项目类别:
-
资助金额:$1.58万
-
财政年份:1997
-
负责人:MICHAEL W THOMPSON
-
依托单位:
海外基金