PAPILLOMAVIRUS TRANSCRIPTIONAL PROGRAM

乳头瘤病毒转录程序

• 批准号: 2463666
• 负责人: C C BAKER
• 金额: --
• 依托单位: DIVISION OF BASIC SCIENCES - NCI
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/2463666
• 关键词: bovine papillomavirus; chemical binding; embryo /fetus tissue /cell culture; genetic promoter element; genetic regulatory element; genetic transcription; human papillomavirus; nucleic acid sequence; posttranscriptional RNA processing; transcription factor; virus RNA; virus genetics; virus replication

The papillomavirus origins of DNA replication and the E6/E7 promoters share overlapping sequences. In addition, transcriptional regulatory proteins have been shown to be required for replication and replication proteins modulate transcription. These regulatory regions have been analyzed in two viruses: human papillomavirus type 16 (HPV-16) and bovine papillomavirus (BPV-1). The HPV-16 long control region (LCR) contains three binding sites for the E2 transcriptional regulatory region. Mutational analysis of these sites demonstrated that there is a direct correlation between the number of E2 binding sites and the amount of DNA replication in transient assays in the SCC-9 squamous cell carcinoma cell line. In these assays the viral E1 and E2 proteins are expressed from separate expression vectors. Mutation of the P97 (E6/E7 promoter) TATA box had minimal effect on replication, indicating that transcription from this region is not required for replication. The corresponding region of BPV-1 contains several E2 binding sites as well as binding sites for cellular transcription factors including SP1, E2F, AP-1, YY-1, and NF-1. Mutations were made in these sites in the context of a P89 (E6/E7) promoter reporter plasmid. The effects of these mutations were assayed by transient transfection in bovine embryo fibroblasts (BEF) in the presence or absence of the E2 protein. We have previously shown that three SP1 sites are required for both basal and transactivated expression. Similar results were seen for the NF-1 site. The YY-1 and AP-1 sites also appear to be important for regulation of P89 transcription. However, we have been unable to demonstrate a role for the E2F site in our assays.

乳头瘤病毒DNA复制的起源和E6/E7启动子