CHARACTERIZATION OF CHRONIC, ASYMPTOMATIC INTESTINAL SHEDDING OF SHIGELLA

志贺氏菌慢性、无症状肠道排出的特征

• 批准号: 2568903
• 负责人: D J KOPECKO
• 金额: --
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/2568903
• 关键词: Peru; Primates; Shigella; United States; bacterial genetics; bactericidal immunity; chronic disease /disorder; clinical research; communicable disease diagnosis; diarrhea; feces analysis; gastrointestinal epithelium; gastrointestinal infection; human tissue; latent bacterial disease; nucleic acid probes; nucleic acid sequence; polymerase chain reaction; tissue /cell culture; virulence

These studies are directed at utilizing specific cloned virulence gene regions of Shigella as DNA probes or their sequence information in polymerase chain reaction assays to detect Shigella in stools. Our early studies showed that DNA probes were very sensitive, quick, and effective in detecting Shigella in the stool of diarrheal disease patients in Peru. In more recent studies utilizing polymerase chain reaction analysis of stools of healthy (i.e. asymptomatic) nonhuman primates and their healthy human caretakers, we have found a high rate of chronic, low level (i.e. below bacteriologic detection) asymptomatic carriage of Shigella. Our specific objectives are to determine (1) if these Shigella have full virulence potential, (2) the exact state in which Shigella are residing in these carriers (e.g. inside mucosal epithelial cells in the colon?), (3) the immune status of the carrier to Shigella, and (4) if this chronic low level carrier state is responsible for maintaining Shigella endemicity in the U.S. where there are now over 25,000 cases per year.

这些研究旨在利用特异性克隆的毒力基因 志贺氏菌的DNA探针或它们的序列信息， 聚合酶链反应检测粪便中的志贺氏菌。我们早期 研究表明，DNA探针非常灵敏、快速和有效 在秘鲁的腹泻病患者粪便中检测志贺氏菌。 在最近的研究中，利用聚合酶链反应分析 健康（即无症状）非人灵长类动物及其健康 人类护理人员，我们发现慢性，低水平（即， 低于细菌学检测）无症状携带志贺氏菌。我们 具体目标是确定（1）这些志贺氏菌是否具有完整的 毒力潜力，（2）志贺氏菌所处的确切状态 在这些载体中（例如在结肠中的粘膜上皮细胞内？）， (3)携带者对志贺氏菌的免疫状态，以及（4）如果这种慢性 低水平携带状态负责维持志贺氏菌 在美国每年有超过25，000例的地方病。