RAPID SCREENING FOR PL-A1 ALLOANTIGEN USING SOLID PHASE MICROPLATE IMMUNOASSAY

使用固相微孔板免疫分析法快速筛选 PL-A1 同种抗原

• 批准号: 2456684
• 金额: --
• 依托单位: CLINICAL CENTER
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/2456684
• 关键词: blood donor; blood group antigens; clinical research; diagnosis quality /standard; genotype; human tissue; immunologic assay /test; isoantigen; mass screening; platelets

Platelet rich plasmas are tested using the Capture-P kit (Immucor). Platelet monolayers in microtiter wells are incubated with anti-PL- A1, washed, and exposed to anti-immunoglobulin G-coated red blood cells. The coated red blood cells adhere to PL-A1 positive platelets but not to PL-A1 negative platelets. Centrifugal sedimentation of unattached red blood cells produces a central button, indicating that platelets are PL-A1 negative. Of 520 donors, 15 (2.88%) tested PL-A1 negative, which correlates well with the reported frequency of PL-A2 in caucasians in the literature. All 15 PL-A1 negative donors were confirmed by allele-specific restriction assays. Confirmatory genotyping was not done on those donors testing PL-A1 positive. The rate of PL-A1 negative donors correlates well with the published rate for caucasians. The assay can be used to screen large numbers of samples. Confirmatory testing can be reserved for sample testing PL-A1 negative by solid phase. Ongoing work involves identifying the rate of "false positives."

使用Capture-P试剂盒（Immucor）检测富血小板血浆。 将微量滴定威尔斯孔中的血小板单层与抗PL- A1，清洗并暴露于抗免疫球蛋白G包被的红血 细胞包被的红细胞粘附于PL-A1阳性血小板 而对PL-A1阴性血小板无明显影响。离心沉淀法 分离的红细胞产生一个中央按钮，表明 血小板是PL-A1阴性的520例献血者中，15例（2.88%）进行了检测 PL-A1阴性，这与报告的 文献中的白种人PL-A2。所有15例PL-A1阴性供体 通过等位基因特异性限制性测定证实。确证性 未对PL-A1检测阳性的供体进行基因分型。的 PL-A1阴性供体的比率与公布的 高加索人的比例。该测定可用于筛选大量 的样本。验证性测试可保留用于样品测试 PL-A1固相阴性。正在进行的工作包括确定 假阳性率。"