ANALYSIS AND SYNTHESIS OF CARBOHYDRATE ANTIGENS OF MYCOBACTERIUM TUBERCULOSIS

结核分枝杆菌碳水化合物抗原的分析与合成

• 批准号: 2575687
• 负责人: V POZSGAY
• 金额: --
• 依托单位: EUNICE KENNEDY SHRIVER NATIONAL INSTITUTE OF CHILD HEALTH & HUMAN DEVELOPMENT
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/2575687
• 关键词: Mycobacterium tuberculosis; bacterial antigens; bacterial polysaccharides; chemical structure function; chemical synthesis; human tissue

Mycobacterium tuberculosis, the causative organism of tuberculosis is responsible for approximately three million death cases annually and the mortality rate of infections caused by multiple drug-resistant strains of this bacterium exceed 80%. M. tuberculosis is coated by a number of polysaccharides of unusual structure. A unique component termed polysaccharide II is composed of alpha, 1,2-linked B-glucose residues. Schemes were designed to isolate this polysaccharide from extracts of M. tuberculosis. We demonstrated that the major monosaccharide components in soluble polysaccharide fractions from Mycobacteria are arabinose, mannose and galactose. A polysaccharide fraction was also identified which contains mannose and glucose in approximately equal amounts. We demonstrated the presence of a glycogen-like polysaccharide as one of the extracellular polysaccharides of Mycobacteria and developed a method for the chemical synthesis of oligosaccharides corresponding to the putative polysaccharide II.

结核分枝杆菌是结核病的病原体， 每年造成大约300万例死亡， 多重耐药菌株感染的死亡率 该菌的产率超过80%。 M.结核病被许多 不寻常结构的多糖。 一种独特的成分， 多糖II由α，1，2-连接的B-葡萄糖残基组成。 设计了从M. 结核 我们证明了主要的单糖成分 在分枝杆菌的可溶性多糖级分中是阿拉伯糖， 甘露糖和半乳糖。 还鉴定了多糖级分 其含有大致等量的甘露糖和葡萄糖。我们 证明了糖原样多糖的存在， 分枝杆菌的胞外多糖，并开发了一种用于 低聚糖的化学合成， 多糖II.