MULTIPLEX RT-PCR BASED TEST FOR HIV-1, HTLVI/II, HCV

基于多重 RT-PCR 的 HIV-1、HTLVI/II、HCV 检测

• 批准号: 2029409
• 负责人: Lucia I. Gonzalez
• 金额: $ 37.93万
• 依托单位: SERACARE BIOSERVICES
• 依托单位国家: 美国
• 财政年份: 1995
• 资助国家: 美国
• 起止时间: 1995-06-01 至 1999-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2029409
• 关键词: deltaretrovirus; diagnosis design /evaluation; diagnostic tests; hepatitis B virus group; human T cell lymphotropic virus type 1; human T cell lymphotropic virus type 2; human immunodeficiency virus 1; human subject; nucleic acid hybridization; polymerase chain reaction; serology /serodiagnosis; uridine triphosphate

Despite the use of highly sensitive serologic tests for screening blood donations for antibodies to HIV-1, HTLV-I/II, HCV and HBV in US blood centers, the delay between the onset of infection and the antibody response creates a "window" period during which the blood may be infectious but seronegative. As a result, a low risk of virus transmission due to contaminated blood products still exists. During PHASEI of this grant, we have developed a Multiplex RT-PCR based capture assay which permits the simultaneous direct detection of HTLV-I/II, HIV-1, HCV and HBV in a single assay. In the course of PHASE II, we propose to further refine this Multiplex RT-PCR assay, and to develop a kit format useful for routine screening of blood products in a clinical lab setting. The assay will be validated on a number of well characterized performance and seroconversion panels for all five viruses and on large numbers (>1,000) of blood samples from normal blood donors. This test will provide a highly sensitive, cost-effective direct measure of infectious virus in blood and help further reduce the antibody-negative "window" period between infectivity and detection.

尽管使用高度敏感的血清学测试来筛选血液