DNA SYNTHESIS AND RECOMBINATION BY HIV DNA POLYMERASE

描述：(改编自调查人员摘要)：应用程序 将研究HIV-1进行链转移的机制。 具体实验包括检测核糖核酸酶H的作用 病毒RT在转移过程中。此外，RT在其中的作用 促进转移反应及其对实验的影响 条件与病毒核衣壳蛋白的反应将是 下定决心。研究人员将确定突变的频率 伴随着同源重组和非同源重组。突变 与转移反应相关联将与特定的 供体模板上的序列特征。链转移的检验 奈韦拉平存在时的效率和诱变将决定 该药物是否改变链转移效率 诱变。耐药突变RT将用于分析 链转移参数和诱变。这些结果将表明 药物治疗或药物诱导的RT突变是否可以促进或 抑制病毒重组。

DESCRIPTION: (Adapted from investigator's abstract): The application will examine the mechanism of strand transfer carried out by HIV-1. Specific experiments include examination of the role of RNAse H of the viral RT in the process of transfer. Additionally, the roles of RT in promoting a transfer reaction and the influence of experimental conditions and viral nucleocapsid protein on the reaction will be determined. The investigator will determine the frequency of mutations accompanying homologous versus nonhomologous recombination. Mutations associated with the transfer reaction will be correlated to specific sequence features on the donor template. Examination of strand transfer efficiency and mutagenesis in the presence of Nevirapine will determine whether that drug alters the efficiency of strand transfer in mutagenesis. Drug resistant mutant RT will be used for analysis of strand transfer parameters and mutagenesis. These results will indicate whether drug treatment or drug induced RT mutations can promote or suppress viral recombination.