INFRARED ANALYSIS OF POLYPEPTIDES AND PROTEINS

多肽和蛋白质的红外分析

• 批准号: 2707112
• 负责人: BAL RAM SINGH
• 金额: $ 0.3万
• 依托单位: UNIVERSITY OF MASSACHUSETTS DARTMOUTH
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-07-15 至 1999-07-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2707112
• 关键词: infrared spectrometry; meeting /conference /symposium; protein structure function; structural biology; travel

DESCRIPTION: An International Symposium on Infrared Analysis of Peptides and Proteins is being organized during the 216th Annual Meeting of American Chemical Society, August 23-27, 1998, Boston, MA. The developments of the last decade in the increased application of IR spectroscopy for peptide and protein analysis have helped understand their physical structure and function correlation. However, the technique is still not a commonly used method for the purpose, and remains an optional method for experts only. Several issues related to protein IR analysis range from instrumentation and sampling methods to theoretical and experimental band assignments and derivations. A symposium exclusively dealing with protein IR is therefore appropriate, and is likely to result in exchange of ideas that could synergistically advance the field. Advancement in the field of structural biology is critical to the advancement of biomedical sciences. Among spectroscopic techniques, IR spectroscopy is perhaps the most versatile and sensitive technique for protein structure analysis, in general, and membrane proteins in particular. However, it has not become as yet common technique for the purpose. The proposed symposium will be a small step in that direction. The following sessions and topics are planned. Infrared Spectroscopy; Instrumentation and Basic Techniques: General introduction and overview of the technique; Interpreting Infrared Spectra of Peptides and Proteins; Infrared spectrometers and protein sampling methods; IR band analysis for the secondary structures of proteins. Advanced Protein IR Techniques: IR spectroscopy of membrane proteins; Infrared circular dichroism of peptide and proteins; Time-resolved infrared spectroscopy of proteins; Two- dimensional IR spectroscopy of proteins; Polarized infrared spectroscopy. Application of Protein Ir Spectroscopy: Analysis of protein-protein interactions using isotope-edited IR spectroscopy; Conformational analysis of synthetic peptides using FTIR spectroscopy-potentials and pitfalls; Application of protein IR spectroscopy for determining molten globule in protein unfolding process.

描述：国际多肽红外分析研讨会 和蛋白质在第216届年度会议期间组织 美国化学学会，1998年8月23-27日，马萨诸塞州波士顿。这个 近十年来信息检索应用领域的新进展 多肽和蛋白质的光谱分析有助于理解它们的 身体结构和功能的相关性。然而，这项技术是 仍然不是用于此目的的常用方法，并且仍然是可选的 方法仅限专家使用。蛋白质红外光谱分析中的几个问题 范围从仪器和采样方法到理论和 实验波段分配和派生。独家举办的研讨会 因此，处理蛋白质IR是适当的，并且很可能导致 以交流能够协同推进该领域的想法。 结构生物学领域的进展对 生物医学科学的进步。在光谱技术中，红外光谱 光谱学可能是最通用和最灵敏的技术 蛋白质结构分析，一般情况下，和膜蛋白 很特别。然而，它还没有成为一种常见的技术 目的。拟议的研讨会将是朝着这个方向迈出的一小步。 计划举行以下会议和主题。红外光谱； 工具和基本技术：一般介绍和概述 技术；解译多肽和蛋白质的红外光谱； 红外光谱仪和蛋白质取样方法.红外波段分析 蛋白质的二级结构。先进的蛋白质红外光谱技术 膜蛋白的光谱；多肽的红外圆二色谱 和蛋白质；蛋白质的时间分辨红外光谱；两个 蛋白质的三维红外光谱；偏振红外光谱。 蛋白质红外光谱技术在蛋白质-蛋白质分析中的应用 使用同位素编辑红外光谱分析的相互作用.构象分析 使用FTIR光谱对合成肽进行分析--潜力和陷阱； 蛋白质红外光谱分析在熔融小球测定中的应用 蛋白质的展开过程。