Comprehensive characterisation of DNA demethylation pathways in vivo

体内 DNA 去甲基化途径的综合表征

• 批准号: DP190103852
• 负责人: A/Prof Ozren Bogdanovic
• 金额: $ 25.97万
• 依托单位: The University of New South Wales
• 依托单位国家: 澳大利亚
• 项目类别: Discovery Projects
• 财政年份: 2019
• 资助国家: 澳大利亚
• 起止时间: 2019-01-01 至 2021-12-31
• 项目状态: 已结题
• 来源: https://dataportal.arc.gov.au/RGS/Web/Grants/DP190103852
• 关键词: Comprehensive; characterisation; DNA; demethylation; pathways

This project aims to provide a better understanding of the roles that DNA methylation plays during embryonic development. DNA methylation is a major regulatory mark present in vertebrate genomes. It is well established that the genomic patterns of DNA methylation are being actively remodelled during vertebrate embryogenesis. Nevertheless, it remains unclear how these events impact gene regulation and embryonic development itself. This project expects to unravel the functional contributions of DNA methylation to vertebrate embryogenesis by using latest cutting-edge genomics techniques. The project will be carried out on the highly tractable zebrafish model system which allows for easy genetic manipulation of the desired sequences. This project aims to provide a better understanding of embryonic development of vertebrates, including humans.

该项目旨在更好地了解DNA甲基化在胚胎发育过程中的作用。DNA甲基化是脊椎动物基因组中的一个主要调控标志。众所周知，DNA甲基化的基因组模式在脊椎动物胚胎发生过程中被积极重塑。然而，目前尚不清楚这些事件如何影响基因调控和胚胎发育本身。本项目希望利用最新的基因组学技术，揭示DNA甲基化对脊椎动物胚胎发生的功能贡献。该项目将在高度易处理的斑马鱼模型系统上进行，该系统允许对所需序列进行简单的遗传操作。该项目旨在更好地了解包括人类在内的脊椎动物的胚胎发育。